Construction of NK cell-conditional Cd226 knockout mice and preliminary investigation of their role in ulcerative colitis.
- Author:
Jianchun LYU
1
;
Zichan GUO
2
;
Yazhen WANG
2
;
Ziyan CHEN
2
;
Zhengxiang ZHANG
3
,
4
;
Lihua CHEN
5
Author Information
1. Yan'an Medical College, Yan'an University, Yan'an 716000; Department of Immunology, Basic Medical Science Academy, Air Force Medical University, Xi'an 710032, China.
2. Department of Immunology, Basic Medical Science Academy, Air Force Medical University, Xi'an 710032, China.
3. Yan'an Medical College, Yan'an University, Yan'an 716000, China. *Corresponding authors, E-mail: zhangzhengxiang168@
4. com.
5. Department of Immunology, Basic Medical Science Academy, Air Force Medical University, Xi'an 710032, China. *Corresponding authors, E-mail: chenlh@fmmu.edu.cn.
- Publication Type:Journal Article
- MeSH:
Animals;
Colitis, Ulcerative/pathology*;
Killer Cells, Natural/metabolism*;
Mice, Knockout;
T Lineage-Specific Activation Antigen 1;
Antigens, Differentiation, T-Lymphocyte/genetics*;
Mice;
Disease Models, Animal;
Mice, Inbred C57BL;
Male
- From:
Chinese Journal of Cellular and Molecular Immunology
2025;41(6):488-494
- CountryChina
- Language:Chinese
-
Abstract:
Objective To generate and characterize natural killer cell (NK cell)-conditional Cd226 gene knockout mice using Cre-loxP technology, and to explore the role of CD226 on NK cells in alleviating intestinal inflammation in a murine model of ulcerative colitis (UC). Methods NK cell-conditional Cd226 gene knockout mice were generated by crossing loxP-flanked Cd226 mice with Ncr1-Cre mice via the Cre-loxP system. Polymerase chain reaction (PCR) and agarose gel electrophoresis were used for genotyping. A UC model was established by dextran sulfate sodium (DSS) induction. Flow cytometry was performed to analyze CD226 expression levels on NK cells and the infiltration of related immune cells in colon tissues. Hematoxylin-eosin (HE) staining was performed to assess the degree of colonic inflammation. Results DNA gel electrophoresis and flow cytometry confirmed the successful generation of NK cell-specific Cd226 knockout mice. After conditional knockout of Cd226 in NK cells, inflammation in the UC mouse model was alleviated. Flow cytometry results showed a reduced proportion of NK cells in peripheral blood and the colon lamina propria, while HE staining demonstrated attenuated inflammatory responses. Conclusion Specific knockout of Cd226 in NK cells mitigates intestinal inflammation in UC mice by reducing NK cell numbers and inhibiting their pro-inflammatory functions.
