Mechanism analysis of platelet activation induced by V. vulnificus hemolysin.
- Author:
Yan WANG
1
;
Zihan FENG
2
;
Yaru WANG
3
;
Shiqing LI
4
;
Xin CHEN
5
;
Jinglin WANG
6
;
Yuan YUAN
7
,
8
Author Information
1. School of Public Health and Health Management, Gannan Medical University, Ganzhou 341000; State Key Laboratory of Pathogen and Biosecurity, Academy of Military Medical Sciences, Beijing 100071, China.
2. Department of Disease Prevention and Control, The No.96110 Hospital of the People's Liberation Army, Yinchuan 750021, China.
3. Hainan Medical Products Administration, Drug Inspection Center in Hainan, Haikou 570216, China.
4. State Key Laboratory of Pathogen and Biosecurity, Academy of Military Medical Sciences, Beijing 100071, China.
5. School of Public Health and Health Management, Gannan Medical University, Ganzhou 341000, China.
6. State Key Laboratory of Pathogen and Biosecurity, Academy of Military Medical Sciences, Beijing 100071, China. *Corresponding authors, E-mail: wjlwjl0801@sina.com.
7. State Key Laboratory of Pathogen and Biosecurity, Academy of Military Medical Sciences, Beijing 100071, China. *Corresponding authors, E-mail: miniminiyuan@
8. com.
- Publication Type:Journal Article
- MeSH:
Animals;
Platelet Activation/drug effects*;
Hemolysin Proteins/pharmacology*;
Vibrio vulnificus/metabolism*;
Mice;
Blood Platelets/drug effects*;
Vibrio Infections/immunology*;
P-Selectin/metabolism*;
Bacterial Proteins;
Female
- From:
Chinese Journal of Cellular and Molecular Immunology
2025;41(2):134-142
- CountryChina
- Language:Chinese
-
Abstract:
Objective To evaluate whether Vibrio vulnificus secreted exotoxin-hemolysin (VVH) can activate platelet, an important blood immune cell, and to explore the possible molecular mechanism of platelet activation by VVH. Methods Transcriptomics and immunohistochemistry were used to analyze whether Vibrio vulnificus infection caused platelet activation in mice. Then, flow cytometry was used to identify whether VVH was the main stimulator of platelet activation. Naturally expressed VVH toxin was purified and prepared. The effects of extracellular and intracellular Ca2+ signal inhibitors on VVH activated platelets were evaluated by flow cytometry and Western blotting. The immune activation effect of VVH in the early stage of Vibrio vulnificus infection was analyzed in vivo. Results VVH was the main stimulator of platelet activation in Vibrio vulnificus culture supernatant. Natural VVH can induce the increase of P-selectin (CD62P) on platelet surface, the formation of platelet-neutrophil complex (PNC), and the release of platelet microvesicles. The activation mechanism may be related to the VVH pore-dependent Ca2+-calmodulin (CaM) -myosin light chain kinase (MLCK) signaling pathway, which led to the release of platelet alpha particles and cascade activation of platelets. In a mouse model of ALD infected by Vibrio vulnificus gavage, VVH was strongly associated with platelet activation. Conclusion This study shows that VVH is an important platelet activating molecule in the early stage of Vibrio vulnificus infection, and its induction of platelet activation may be related to the pathogenic process.
