Progress in investigating astrocyte heterogeneity after spinal cord injury based on single-cell sequencing technology.
10.12200/j.issn.1003-0034.20240352
- Author:
Lei DU
1
;
Yan-Jun ZHANG
2
;
Tie-Feng GUO
2
;
Lin-Zhao LUO
2
;
Ping-Yi MA
2
;
Jia-Ming LI
2
;
Sheng TAN
1
Author Information
1. Gansu University of Traditional Chinese Medicine, Lanzhou 730000, Gansu, China.
2. Gansu Provincial Hospital of Traditional Chinese Medicine, Lanzhou 730050, Gansu, China.
- Publication Type:English Abstract
- Keywords:
Astrocytes;
Neurological recovery;
Pathological mechanism;
Single cell sequencing technology;
Spinal cord injury
- MeSH:
Spinal Cord Injuries/metabolism*;
Astrocytes/cytology*;
Single-Cell Analysis/methods*;
Humans;
Animals;
Transcriptome;
Nerve Regeneration
- From:
China Journal of Orthopaedics and Traumatology
2025;38(5):544-548
- CountryChina
- Language:Chinese
-
Abstract:
In recent years, the study of single-cell transcriptome sequencing technology in the heterogeneity of astrocytes (astrocytes) after spinal cord injury (SCI) has provided new perspectives on post-traumatic nerve regeneration and repair. To provide a review on the research progress of single-cell sequencing technology in astrocytes after spinal cord injury (SCI), and to more comprehensively and deeply elaborate the application of single-cell sequencing technology in the field of astrocytes after SCI. Single-cell sequencing technology can analyse the transcriptomes of individual cells in a high-throughput manner, thus revealing fine differences in cell types and states. By using single-cell sequencing technology, the heterogeneity of astrocytes after SCI and their association with nerve regeneration and repair were revealed. In conclusion, the application of single-cell sequencing technology provides an important tool to reveal the heterogeneity of astrocytes after SCI, to further explore the mechanisms of astrocytes in SCI, and to develop intervention strategies targeting their regulatory mechanisms in order to improve the therapeutic efficacy of SCI. The discovery of changes in astrocyte transcriptome dynamics has improved researchers' understanding of spinal cord injury lesion progression and provided new insights into the treatment of spinal cord injury at different time points. To date, all of these findings need to be validated by more basic research and sufficient clinical trials. In the future, single-cell sequencing technology, through interdisciplinary collaboration with bioinformatics, computer science, tissue engineering, and clinical medicine, is expected to open a new window for the treatment of spinal cord injury.
