Effect of autologous osteochondral tissue and periosteum transplantation on tendon-bone healing of rotator cuff in rabbits.
10.7507/1002-1892.202409094
- Author:
Sen FANG
1
;
Mingtao ZHANG
1
;
Xudong YANG
1
;
Cairang DAOJI
1
;
Mingchun LI
1
;
Zhixuan NIAN
1
;
Junwen LIANG
1
;
Xiangdong YUN
1
Author Information
1. Department of Orthopedics, the Second Hospital of Lanzhou University, Gansu Lanzhou, 730000, P. R. China.
- Publication Type:Journal Article
- Keywords:
Rotator cuff injury;
autogenous transplantation;
osteochondral tissue;
periosteum;
rabbit;
tendon-bone healing
- MeSH:
Animals;
Rabbits;
Male;
Wound Healing;
Transplantation, Autologous;
Periosteum/transplantation*;
Rotator Cuff Injuries;
Rotator Cuff/surgery*;
Tendons/surgery*;
Biomechanical Phenomena;
Chondrocytes/transplantation*;
Tendon Injuries/surgery*;
Tensile Strength
- From:
Chinese Journal of Reparative and Reconstructive Surgery
2025;39(2):187-192
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To investigate the effect of autologous osteochondral tissue and periosteum transplantation on tendon-bone healing of rotator cuff in rabbits.
METHODS:Twenty-four male New Zealand white rabbits were randomly divided into autologous osteochondral tissue and periosteum transplantation group (experimental group, n=12) and simple suture group (control group, n=12). Both groups were subjected to acute supraspinatus tendon injury and repaired with corresponding techniques. At 4, 8, and 12 weeks after operation, 4 specimens from each group were taken from the right shoulder joint for histological examination (HE staining, Masson staining, and Safranin O-fast green staining), and the left shoulder was subjected to biomechanical tests (maximum tensile load and stiffness).
RESULTS:Both groups of animals survived until the completion of the experiment after operation. At 4 weeks after operation, both groups showed less collagen fibers and disorder at the tendon-bone junction. At 8 weeks, both groups showed reduced inflammation at the tendon-bone junction, with more organized and denser collagen fibers and chondrocytes. The experimental group showed better results than the control group. At 12 weeks, the experimental group showed typical tendon-bone transition structure, with increased generation of collagen fibers and chondrocytes, and the larger cartilage staining area. Both groups showed an increase in maximum tensile load and stiffness over time ( P<0.05). The stiffness at 4 weeks and the maximum tensile load at 4, 8, and 12 weeks in the experimental group were superior to control group, and the differences were significant ( P<0.05). There was no significant difference in stiffness at 8, 12 weeks between the two groups ( P>0.05).
CONCLUSION:Autologous osteochondral tissue and periosteum transplantation can effectively promote the fiber and cartilage regeneration at the tendon-bone junction of rotator cuff and improve the biomechanical effect of shoulder joint in rabbits.
