Study on the protective effects of resveratrol on the liver of hindlimb-unloaded rats.
10.7507/1001-5515.202405011
- Author:
Yingying XUAN
1
;
Yutian YANG
1
;
Hanqin TANG
1
;
Zhihui MA
1
;
Liang LI
1
;
Dongshuai SHEN
1
;
Mei ZHANG
1
;
Keming CHEN
1
Author Information
1. Basic Medical Laboratory, The 940 Hospital of Joint Logistic Support Hospital, Lanzhou 730050, P. R. China.
- Publication Type:Journal Article
- Keywords:
Hepatoprotective effect;
Hindlimp unloading;
Inflammatory factors;
Oxidative stress;
Resveratrol
- MeSH:
Animals;
Resveratrol;
Male;
Liver/pathology*;
Rats, Sprague-Dawley;
Rats;
Hindlimb Suspension;
Oxidative Stress/drug effects*;
Phosphatidylinositol 3-Kinases/metabolism*;
Proto-Oncogene Proteins c-akt/metabolism*;
Stilbenes/pharmacology*;
bcl-2-Associated X Protein/metabolism*;
Proto-Oncogene Proteins c-bcl-2/metabolism*;
Apoptosis/drug effects*
- From:
Journal of Biomedical Engineering
2024;41(6):1250-1256
- CountryChina
- Language:Chinese
-
Abstract:
This study aims to investigate the protective effect of resveratrol against liver injury in hindlimb unloading rats. Thirty 2-month-old male SD rats were randomly divided into normal group (Control), hindlimb unloading model group (Model), and hindlimb unloading+resveratrol administration group (Model+Res). The Model + Res group was injected intraperitoneally with 30 mg/kg of resveratrol, and the Control and Model groups were injected intraperitoneally with an equal volume of 0.9% NaCl. Liver tissues were collected after 28 days and analyzed for oxidative stress, inflammatory factors, energy metabolism indices, Na +-K +-ATPase and Ca 2+-Mg 2+-ATPase activity, and morphological changes were observed by hematoxylin-eosin staining. The protein expression levels of Bax, Bcl-2, p-PI3K, PI3K, p-AKT, and AKT were detected by Western blotting. Compared with the Control group, hepatocytes in the Model group showed swelling, abnormal morphology, nuclear consolidation, and cell membrane disruption. Oxidative stress, inflammatory factor levels, hepatic glycogen accumulation, and energy metabolism were increased in the liver tissues of the Model group, while resveratrol treatment significantly reversed these changes. The results of Western blotting showed that resveratrol significantly reduced the expression of Bax and increased the expression levels of Bcl-2, and the proteins of p-PI3K/PI3K and p-AKT/AKT expression levels. It is suggested that 28 days of hindlimb unloading treatment could lead to liver tissue injury in rats, which is manifested as oxidative stress, inflammatory response, energy metabolism disorder and increased apoptosis level, and resveratrol has a certain mitigating effect on this.
