Quality evaluation of Xinjiang Rehmannia glutinosa and Rehmannia glutinosa based on fingerprint and multi-component quantification combined with chemical pattern recognition.
10.19540/j.cnki.cjcmm.20250427.101
- Author:
Pan-Ying REN
1
;
Wei ZHANG
2
;
Xue LIU
1
;
Juan ZHANG
1
;
Cheng-Fu SU
1
;
Hai-Yan GONG
1
;
Chun-Jing YANG
1
;
Jing-Wei LEI
1
;
Su-Qing ZHI
3
;
Cai-Xia XIE
1
Author Information
1. Collaborative Innovation Center of Research and Development on the Whole Industry Chain of Yu-Yao, Henan University of Chinese Medicine Zhengzhou 450046, China.
2. Zhengzhou Health Vocational College Zhengzhou 450046, China.
3. Jiaozuo Jiu Ru Huai Qing Chinese Medicine Intangible Cultural Heritage Research Institute Jiaozuo 454350, China.
- Publication Type:Journal Article
- Keywords:
Rehmannia glutinosa;
Xinjiang Rehmannia glutinosa;
chemical pattern recognition;
content determination;
entropy weight TOPSIS method;
fingerprint
- MeSH:
Rehmannia/classification*;
Drugs, Chinese Herbal/chemistry*;
Chromatography, High Pressure Liquid/methods*;
Quality Control
- From:
China Journal of Chinese Materia Medica
2025;50(16):4630-4640
- CountryChina
- Language:Chinese
-
Abstract:
The differences in chemical quality characteristics between Xinjiang Rehmannia glutinosa and R. glutinosa were analyzed to provide a theoretical basis for the introduction and quality control of R. glutinosa. In this study, the high performance liquid chromatography(HPLC) fingerprints of 6 batches of Xinjiang R. glutinosa and 10 batches of R. glutinosa samples were established. The content of iridoid glycosides, phenylethanoid glycosides, monosaccharides, oligosaccharides, and polysaccharides in Xinjiang R. glutinosa and R. glutinosa was determined by high performance liquid chromatography-diode array detection(HPLC-DAD), high performance liquid chromatography-evaporative light scattering detection(HPLC-ELSD), and ultraviolet-visible spectroscopy(UV-Vis). The determination results were analyzed with by chemical pattern recognition and entropy weight TOPSIS method. The results showed that there were 19 common peaks in the HPLC fingerprints of the 16 batches of R. glutinosa, and catalpol, aucubin, rehmannioside D, rehmannioside A, hydroxytyrosol, leonuride, salidroside, cistanoside A, and verbascoside were identified. Hierarchical cluster analysis(HCA) and principal component analysis(PCA) showed that Qinyang R. glutinosa, Mengzhou R. glutinosa, and Xinjiang R. glutinosa were grouped into three different categories, and eight common components causing the chemical quality difference between Xinjiang R. glutinosa and R. glutinosa in Mengzhou and Qinyang of Henan province were screened out by orthogonal partial least squares discriminant analysis(OPLS-DA). The results of content determination showed that there were glucose, sucrose, raffinose, stachyose, polysaccharides, and nine glycosides in Xinjiang R. glutinosa and R. glutinosa samples, and the content of catalpol, rehmannioside A, leonuride, cistanoside A, verbascoside, sucrose, and glucose was significantly different between Xinjiang R. glutinosa and R. glutinosa. The analysis with entropy weight TOPSIS method showed that the comprehensive quality of R. glutinosa in Mengzhou and Qinyang of Henan province was better than that of Xinjiang R. glutinosa. In conclusion, the types of main chemical components of R. glutinosa and Xinjiang R. glutinosa were the same, but their content was different. The chemical quality of R. glutinosa was better than Xinjiang R. glutinosa, and other components in R. glutinosa from two producing areas and their effects need further study.
