Differences in intestinal absorption characteristics of Rubus multibracteatus extract in normal and inflammatory pain model rats by in-vitro everted intestine sac method.
10.19540/j.cnki.cjcmm.20250509.202
- Author:
Ming-Li BAO
1
;
Qing ZHANG
2
;
Yang JIN
3
;
Yi CHEN
3
;
Jian-Qing PENG
3
;
Si-Ying CHEN
3
;
Zhi-Jie MA
4
;
Jian LIAO
1
;
Jing HUANG
3
;
Zi-Peng GONG
5
Author Information
1. College of Stomatology of Guizhou Medical University/Stomatological Hospital of Guizhou Medical University Guiyang 550004, China.
2. State Key Laboratory of Discovery and Utilization of Functional Components in Traditional Chinese Medicine,Engineering Research Center for the Development and Application of Ethnic Medicine and Traditional Chinese Medicine,School of Pharmacy, Guizhou Medical University Guiyang 561113, China Department of Pharmacy, Guizhou Branch of Shanghai Children's Medical Center, Shanghai Jiao Tong University School of Medicine Guiyang 550081, China.
3. State Key Laboratory of Discovery and Utilization of Functional Components in Traditional Chinese Medicine,Engineering Research Center for the Development and Application of Ethnic Medicine and Traditional Chinese Medicine,School of Pharmacy, Guizhou Medical University Guiyang 561113, China.
4. Pharmacy Department, Beijing Ditan Hospital,Capital Medical University Beijing 100015, China.
5. College of Stomatology of Guizhou Medical University/Stomatological Hospital of Guizhou Medical University Guiyang 550004, China State Key Laboratory of Discovery and Utilization of Functional Components in Traditional Chinese Medicine,Engineering Research Center for the Development and Application of Ethnic Medicine and Traditional Chinese Medicine,School of Pharmacy, Guizhou Medical University Guiyang 561113, China.
- Publication Type:Journal Article
- Keywords:
Rubus multibracteatus extract;
absorption;
inflammatory pain model rat;
isolated everted intestinal sac model
- MeSH:
Animals;
Rats;
Intestinal Absorption/drug effects*;
Male;
Rats, Sprague-Dawley;
Drugs, Chinese Herbal/metabolism*;
Disease Models, Animal;
Pain/metabolism*;
Intestines/drug effects*;
Intestinal Mucosa/metabolism*
- From:
China Journal of Chinese Materia Medica
2025;50(16):4690-4704
- CountryChina
- Language:Chinese
-
Abstract:
This study compared the differences in intestinal absorption characteristics of eleven active components in Rubus multibracteatus(RM) extract(protocatechuic acid, tiliroside, scutellarin, luteoloside, astragalin, epicatechin, catechin, xanthotoxin, p-coumaric acid, caffeic acid, and apigenin-7-O-glucuronide) between normal rats and inflammatory pain model rats using the in-vitro everted intestinal sac model. The RM extract was administered at absorption concentrations of 25.0, 50.0, and 100.0 mg·mL~(-1). The contents of the eleven components in intestinal absorption solution samples were quantified by ultra-performance liquid chromatography-tandem mass spectrometry(UPLC-MS/MS), and their cumulative absorption(Q) and absorption rate constant(K_a) were calculated to evaluate the absorption characteristics of these components in normal rats and inflammatory pain model rats. The results show that except for catechin, epicatechin, and caffeic acid, the cumulative absorption-time curves of the other eight components(protocatechuic acid, tiliroside, scutellarin, luteoloside, astragalin, xanthotoxin, p-coumaric acid, and apigenin-7-O-glucuronide) exhibit an upward trend without saturation, with correlation coefficients(R~2) all > 0.9, indicating linear absorption. However, the overall absorption of all components is not dose-dependent with increasing concentration, suggesting that their absorption mechanisms are not solely passive diffusion. In both normal and model rats, the jejunum shows the highest absorption for all components except xanthotoxin. The overall absorption of seven components(excluding protocatechuic acid, caffeic acid, apigenin-7-O-glucuronide, and luteoloside) in normal rats is better than that in model rats across all intestinal segments. These findings indicate that the pathological state of inflammatory pain alters the intestinal absorption of RM extract, and its mechanism needs further investigation.
