Development of intelligent equipment for rapid microbial detection of Atractylodis Macrocephalae Rhizoma decoction pieces based on measurement technology for traditional Chinese medicine manufacturing.
10.19540/j.cnki.cjcmm.20250418.301
- Author:
Yang LIU
1
;
Wu-Zhen QI
2
;
Yu-Tong WU
3
;
Shan-Xi ZHU
3
;
Xiao-Jun ZHAO
3
;
Qia-Tong XIE
3
;
Yu-Feng GUO
3
;
Jing ZHAO
3
;
Nan LI
2
;
Shi-Jun WANG
4
;
Qi-Hui SUN
1
;
Zhi-Sheng WU
5
Author Information
1. Shandong University of Traditional Chinese Medicine Ji'nan 250355, China.
2. School of Chinese Materia Medica, Beijing University of Chinese Medicine Beijing 102488, China Engineering Research Center for Pharmaceutics of Chinese Materia Medica and New Drug Development, Ministry of Education Beijing 102488, China.
3. School of Chinese Materia Medica, Beijing University of Chinese Medicine Beijing 102488, China.
4. People's Hospital Affiliated to Fujian University of Traditional Chinese Medicine Fuzhou 350004, China.
5. Shandong University of Traditional Chinese Medicine Ji'nan 250355, China School of Chinese Materia Medica, Beijing University of Chinese Medicine Beijing 102488, China Engineering Research Center for Pharmaceutics of Chinese Materia Medica and New Drug Development, Ministry of Education Beijing 102488, China.
- Publication Type:English Abstract
- Keywords:
Atractylodis Macrocephalae Rhizoma decoction pieces;
intelligent equipment;
measurement technology for traditional Chinese medicine manufacturing;
microorganism;
rapid detection
- MeSH:
Drugs, Chinese Herbal/analysis*;
Atractylodes/microbiology*;
Rhizome/microbiology*;
Biosensing Techniques/methods*;
Medicine, Chinese Traditional;
Colorimetry/instrumentation*;
Quality Control
- From:
China Journal of Chinese Materia Medica
2025;50(16):4610-4618
- CountryChina
- Language:Chinese
-
Abstract:
Microbial detection and control of traditional Chinese medicine(TCM) decoction pieces are crucial for the quality control of TCM preparations. It is also a key area of research in the measurement technology and equipment development for TCM manufacturing. Guided by TCM manufacturing measurement methodologies, this study presented a design of a novel portable microbial detection device, using Atractylodis Macrocephalae Rhizoma decoction pieces as a demonstration. Immunomagnetic separation technology was employed for specific isolation and labeling of target microorganisms. Enzymatic signal amplification was utilized to convert weak biological signals into colorimetric signals, constructing an optical biosensor. A self-developed smartphone APP was further applied to analyze the colorimetric signals and quantify target concentrations. A portable and automated detection system based on Arduino microcontroller was developed to automatically perform target microbial separation/extraction, as well as mimetic enzyme labeling and catalytic reactions. The developed equipment specifically focuses on the rapid and quantitative microbial analysis of TCM active pharmaceutical ingredients, intermediates in TCM manufacturing, and final TCM products. Experimental results demonstrate that the equipment could detect Salmonella in samples within 2 h, with a detection limit as low as 5.1 × 10~3 CFU·mL~(-1). The equipment enables the rapid detection of microorganisms in TCM decoction pieces, providing a potential technical solution for on-site rapid screening of microbial contamination indicators in TCM. It has broad application prospects in measurement technology for TCM manufacturing and offers strong technical support for the modernization, industrialization, and intelligent development of TCM.
