Identification and expression analysis of B3 gene family in Panax ginseng.
10.19540/j.cnki.cjcmm.20250426.101
- Author:
Yu-Long WANG
1
;
Ai-Min WANG
1
;
Jing-Hui YU
1
;
Si-Zhang LIU
1
;
Ge JIN
1
;
Kang-Yu WANG
1
;
Ming-Zhu ZHAO
1
;
Yi WANG
1
;
Mei-Ping ZHANG
1
Author Information
1. College of Life Science, Jilin Agricultural University Changchun 130118, China Research Center for Ginseng Genetic Resources Development and Utilization, Jilin Province,Jilin Agricultural University Changchun 130118, China.
- Publication Type:Journal Article
- Keywords:
B3 transcription factor;
Panax ginseng;
ginsenoside biosynthesis;
methyl jasmonate
- MeSH:
Panax/metabolism*;
Gene Expression Regulation, Plant;
Plant Proteins/metabolism*;
Ginsenosides/biosynthesis*;
Multigene Family;
Phylogeny
- From:
China Journal of Chinese Materia Medica
2025;50(16):4593-4609
- CountryChina
- Language:Chinese
-
Abstract:
Panax ginseng as a perennial herb of Araliaceae, exhibits pharmacological effects such as central nervous system stimulation, anti-tumor properties, and cardiovascular and cerebrovascular protection. The B3 gene family plays a crucial role in growth and development, antioxidant activity, stress resistance, and secondary metabolism regulation of plants and has been extensively studied in various plants. However, the identification and analysis of the B3 gene family in P. ginseng have not been reported. In this study, a total of 145 B3 genes(PgB3s) with complete open reading frames(ORF) were identified from P. ginseng and classified into five subfamilies based on domain types. Through correlation analysis with ginsenoside content, SNP/InDels analysis, and interaction analysis with key enzyme genes, 15 PgB3 transcripts were found to be significantly correlated with ginsenoside content and exhibited a close interaction network with key enzyme genes involved in ginsenoside biosynthesis, which indicated that these genes may participate in the regulation of ginsenoside biosynthesis. Additionally, this study found that PgB3 genes exhibited induced expression in response to methyl jasmonate(MeJA) stress, which aligned with the presence of abundant stress response elements in their promoters, confirming the important role of the B3 gene family in P. ginseng in stress resistance. The results of this study revealed the potential functions of PgB3 genes in ginsenoside biosynthesis and stress response, providing a significant theoretical basis for further research on the functions of PgB3 genes and their regulatory mechanisms.
