Quality evaluation of Bidentis Herba derived from different original plants based on HPLC fingerprints, characteristic chromatograms, multi-component content determination combined with chemical pattern recognition.
10.19540/j.cnki.cjcmm.20250520.201
- Author:
Guo-Li SHI
1
;
Yun MA
2
;
Feng-Xia SHEN
2
;
Han-Wen DU
3
;
Cong-Min LIU
3
;
Rui-Xia WEI
2
;
Yan-Fang LI
2
;
Jian-Wei FAN
4
;
Yong-Xia GUAN
4
Author Information
1. Shandong University of Traditional Chinese Medicine Ji'nan 250355, China State Key Laboratory of Integration and Innovation of Classic Formula and Modern Chinese Medicine Linyi 276006, China.
2. State Key Laboratory of Integration and Innovation of Classic Formula and Modern Chinese Medicine Linyi 276006, China Lunan Pharmaceutical Group Co., Ltd. Linyi 276006, China Shandong Engineering Research Center of Generic Manufacture Technology of Traditional Chinese Medicine,Lunan Hope Pharmaceutical Co., Ltd. Linyi 276006, China.
3. Lunan Pharmaceutical Group Co., Ltd. Linyi 276006, China.
4. Shandong University of Traditional Chinese Medicine Ji'nan 250355, China State Key Laboratory of Integration and Innovation of Classic Formula and Modern Chinese Medicine Linyi 276006, China Lunan Pharmaceutical Group Co., Ltd. Linyi 276006, China Shandong Engineering Research Center of Generic Manufacture Technology of Traditional Chinese Medicine,Lunan Hope Pharmaceutical Co., Ltd. Linyi 276006, China.
- Publication Type:English Abstract
- Keywords:
B.biternata;
Bidens bipinnata;
characteristic chromatogram;
chemical pattern recognition;
content determination;
fingerprint;
quality control
- MeSH:
Chromatography, High Pressure Liquid/methods*;
Drugs, Chinese Herbal/chemistry*;
Quality Control;
Bidens/chemistry*
- From:
China Journal of Chinese Materia Medica
2025;50(15):4284-4292
- CountryChina
- Language:Chinese
-
Abstract:
This study established the HPLC fingerprints, characteristic chromatograms, and a multi-component content determination method for Bidens bipinnata and B. biternata. The chemical pattern recognition analysis was then employed to clarify the characteristic indexes of quality differences between the two original plants of Bidentis Herba, providing a reference for establishing the quality standards of Bidentis Herba. HPLC was launched on an Agilent Poroshell 120 EC-C_(18) chromatographic column(4.6 mm×250 mm, 4 μm) by gradient elution with a mobile phase of 0.1% aqueous phosphoric acid-acetonitrile at a flow rate of 0.7 mL·min~(-1), detection wavelength of 270 nm, column temperature of 25 ℃, and an injection volume of 5 μL. The similarity between the fingerprints of 18 batches of Bidentis Herba samples and the common pattern(R) ranged from 0.572 to 0.933. A total of 23 chromatographic peaks were calibrated. Through comparison with the reference substances, six components(neochlorogenic acid, chlorogenic acid, isochlorogenic acid A, isochlorogenic acid B, rutin, and hyperoside) were identified and subjected to quantitative analysis. The characteristic fingerprints of B. bipinnata and B. biternata were calibrated with 20 and 17 characteristic peaks, respectively. Among them, peaks 8, 9, 22, and 23 were the characteristic peaks of B. bipinnata, and peak 7 was the characteristic peak of B. biternata, which can be used to distinguish the two original plants of Bidentis Herba. The relative standard deviation of the content of the above-mentioned six components ranged from 36% to 123%. The cluster analysis, principal component analysis, and orthogonal partial least squares-discriminant analysis(OPLS-DA) classified the 18 batches of Bidentis Herba samples into two categories. Additionally, through the analysis of variable importance in projection(VIP) under OPLS-DA, three characteristic indexes, rutin, isochlorogenic acid A, and isochlorogenic acid B, were identified. The analytical method established in this study can comprehensively evaluate the consistency of Bidentis Herba samples derived from different original plants, specifically identify the differential components between them, and effectively distinguish the two original plants of Bidentis Herba, providing a basis for the differentiation between different original plants and the quality control of Bidentis Herba.
