Identification and expression analysis of AP2/ERF family members in Lonicera macranthoides.
10.19540/j.cnki.cjcmm.20250414.102
- Author:
Si-Min ZHOU
1
;
Mei-Ling QU
1
;
Juan ZENG
1
;
Jia-Wei HE
1
;
Jing-Yu ZHANG
1
;
Zhi-Hui WANG
2
;
Qiao-Zhen TONG
3
;
Ri-Bao ZHOU
4
;
Xiang-Dan LIU
4
Author Information
1. School of Pharmacy, Hunan University of Chinese Medicine Changsha 410208, China.
2. School of Pharmacy, Hunan University of Chinese Medicine Changsha 410208, China Key Laboratory of Germplasm Resources and Standardized Planting of Hunan Large-scale Genuine Medicinal Materials Changsha 410208, China.
3. School of Pharmacy, Hunan University of Chinese Medicine Changsha 410208, China Key Laboratory of Germplasm Resources and Standardized Planting of Hunan Large-scale Genuine Medicinal Materials Changsha 410208, China Key Laboratory of Traditional Chinese Medicine Modernization Research in General Colleges and Universities of Hunan Province Changsha 410208, China.
4. School of Pharmacy, Hunan University of Chinese Medicine Changsha 410208, China Key Laboratory of Germplasm Resources and Standardized Planting of Hunan Large-scale Genuine Medicinal Materials Changsha 410208, China Key Laboratory of Traditional Chinese Medicine Modernization Research in General Colleges and Universities of Hunan Province Changsha 410208, China Hunan Engineering Research Center of Standardization and Function of Chinese Herbal Decoction Pieces Changsha 410208, China.
- Publication Type:Journal Article
- Keywords:
AP2/ERF;
Lonicera macranthoides;
bioinformatics;
flowers;
transcription factor
- MeSH:
Plant Proteins/chemistry*;
Gene Expression Regulation, Plant;
Transcription Factors/chemistry*;
Lonicera/classification*;
Flowers/metabolism*;
Phylogeny;
Gene Expression Profiling;
Multigene Family
- From:
China Journal of Chinese Materia Medica
2025;50(15):4248-4262
- CountryChina
- Language:Chinese
-
Abstract:
The AP2/ERF transcription factor family is a class of transcription factors widely present in plants, playing a crucial role in regulating flowering, flower development, flower opening, and flower senescence. Based on transcriptome data from flower, leaf, and stem samples of two Lonicera macranthoides varieties, 117 L. macranthoides AP2/ERF family members were identified, including 14 AP2 subfamily members, 61 ERF subfamily members, 40 DREB subfamily members, and 2 RAV subfamily members. Bioinformatics and differential gene expression analyses were performed using NCBI, ExPASy, SOMPA, and other platforms, and the expression patterns of L. macranthoides AP2/ERF transcription factors were validated via qRT-PCR. The results indicated that the 117 LmAP2/ERF members exhibited both similarities and variations in protein physicochemical properties, AP2 domains, family evolution, and protein functions. Differential gene expression analysis revealed that AP2/ERF transcription factors were primarily differentially expressed in the flowers of the two L. macranthoides varieties, with the differentially expressed genes mainly belonging to the ERF and DREB subfamilies. Further analysis identified three AP2 subfamily genes and two ERF subfamily genes as potential regulators of flower development, two ERF subfamily genes involved in flower opening, and two ERF subfamily genes along with one DREB subfamily gene involved in flower senescence. Based on family evolution and expression analyses, it is speculated that AP2/ERF transcription factors can regulate flower development, opening, and senescence in L. macranthoides, with ERF subfamily genes potentially serving as key regulators of flowering duration. These findings provide a theoretical foundation for further research into the specific functions of the AP2/ERF transcription factor family in L. macranthoides and offer important theoretical insights into the molecular mechanisms underlying floral phenotypic differences among its varieties.
