Effects of alcoholic extract of Gnaphalium affine on oxidative stress and intestinal flora in rats with chronic obstructive pulmonary disease.
10.19540/j.cnki.cjcmm.20250318.702
- Author:
Da-Huai LIN
1
;
Xiang-Li YE
2
;
Guo-Hong YAN
3
;
Kai-Ge WANG
1
;
Yu-Qin ZHANG
1
;
Huang LI
1
Author Information
1. School of Pharmacy,Fujian University of Traditional Chinese Medicine Fuzhou 350122,China.
2. Pulmonary and Critical Care Medicine,Fujian Medical University Union Hospital Fuzhou 350001, China.
3. Department of Pharmacy,the Affiliated People's Hospital of Fujian University of Traditional Chinese Medicine Fuzhou 350004,China.
- Publication Type:Journal Article
- Keywords:
Gnaphalium affine;
chronic obstructive pulmonary disease;
homeostasis in intestinal flora;
oxidative stress pathway
- MeSH:
Animals;
Oxidative Stress/drug effects*;
Pulmonary Disease, Chronic Obstructive/genetics*;
Rats;
Male;
Gastrointestinal Microbiome/drug effects*;
Rats, Sprague-Dawley;
Drugs, Chinese Herbal/administration & dosage*;
NF-E2-Related Factor 2/metabolism*;
Humans;
Lung/metabolism*
- From:
China Journal of Chinese Materia Medica
2025;50(15):4110-4119
- CountryChina
- Language:Chinese
-
Abstract:
The efficacy mechanism of the alcoholic extract of Gnaphalium affine was investigated by observing its influence on oxidative stress and intestinal flora in rats modeled for chronic obstructive pulmonary disease(COPD). UPLC-MS was used to evaluate the quality of the alcoholic extract of G. affine, and 72 rats were randomly divided into six groups, with COPD models established in five groups by cigarette smoke combined with airway drip lipopolysaccharide, and the rats were given the positive drug of Danlong Oral Solution, as well as low-, medium-, and high-doses alcoholic extract of G. affine, respectively. After two weeks of continuous gastric gavage, the body weights and general morphology observations were performed; HE staining and Masson staining were used to verify the effects of the alcoholic extract of G. affine on alveolar inflammation and collagen deposition area in COPD rats; the oxidative stress indexes CAT and GSH in serum and SOD and MDA in lung tissue of the rats were measured, and the mRNA expression of HO-1, Nrf2, and NQO1 were determined by qRT-PCR. The protein expressions of HO-1, Nrf2, and NQO1 were determined by the Western blot method, and the mechanism by which the alcoholic extract of G. affine affected oxidative stress in COPD rats was explored. Finally, the influence of G. affine on the changes in intestinal flora caused by COPD was studied by 16S rRNA sequencing. The results showed that a total of 121 chemical components were identified by UPLC-MS, including 70 positive and 51 negative ion modes. In animal experiments, it was found that the alcoholic extracts of G. affine were able to reduce the percentage of collagen deposition, affect the oxidative stress indexes such as CAT, GSH, SOD, MDA, as well as the mRNA and protein expression of Nrf2, HO-1, and NQO1. The 16S rRNA sequencing results showed an increase in the level of Lactobacillales and a decrease in the level of Desulfovibrio and Desulfovibrionales, suggesting that the alcoholic extracts of G. affine could reverse the changes in intestinal flora caused by COPD. In conclusion, the alcoholic extracts of G. affine may exert anti-COPD effects by affecting the oxidative stress pathway and modulating the changes in intestinal flora.
