Effects of combined use of active ingredients in Buyang Huanwu Decoction on oxygen-glucose deprivation/reglucose-reoxygenation-induced inflammation and oxidative stress of BV2 cells.
10.19540/j.cnki.cjcmm.20250214.701
- Author:
Tian-Qing XIA
1
;
Ying CHEN
1
;
Jian-Lin HUA
1
;
Qin SU
2
;
Cun-Yan DAN
1
;
Meng-Wei RONG
1
;
Shi-Ning GE
3
;
Hong GUO
3
;
Bao-Guo XIAO
4
;
Jie-Zhong YU
5
;
Cun-Gen MA
6
;
Li-Juan SONG
7
Author Information
1. Shanxi University of Chinese Medicine, Key Laboratory of Benefiting Qi and Activating Blood Circulation to Treat Multiple Sclerosis (National Administration of Traditional Chinese Medicine),Neurobiology Research Center Jinzhong 030619, China.
2. Shanxi University of Chinese Medicine, Key Laboratory of Benefiting Qi and Activating Blood Circulation to Treat Multiple Sclerosis (National Administration of Traditional Chinese Medicine),Neurobiology Research Center Jinzhong 030619, China Institute of Brain Science,Shanxi Datong University, Datong City Key Laboratory of Molecular Cellular Immunology Datong 037009, China.
3. State Key Laboratory of Component-based Chinese Medicine, Tianjin University of Traditional Chinese Medicine Tianjin 301617, China.
4. State Key Laboratory of Medical Neurobiology, Neurology Department of Huashan Hospital, Fudan University Shanghai 200025, China.
5. Shanxi University of Chinese Medicine, Key Laboratory of Benefiting Qi and Activating Blood Circulation to Treat Multiple Sclerosis (National Administration of Traditional Chinese Medicine),Neurobiology Research Center Jinzhong 030619, China Institute of Brain Science,Shanxi Datong University, Datong City Key Laboratory of Molecular Cellular Immunology Datong 037009, China the Fifth People's Hospital of Datong City Datong 037009, China.
6. Shanxi University of Chinese Medicine, Key Laboratory of Benefiting Qi and Activating Blood Circulation to Treat Multiple Sclerosis (National Administration of Traditional Chinese Medicine),Neurobiology Research Center Jinzhong 030619, China Neurosurgery Department of Sinopharm Tongmei General Hospital,Key Laboratory of Neurological Disease Prevention and Control of Health Commission of Shanxi Province Datong 037003, China.
7. Shanxi University of Chinese Medicine, Key Laboratory of Benefiting Qi and Activating Blood Circulation to Treat Multiple Sclerosis (National Administration of Traditional Chinese Medicine),Neurobiology Research Center Jinzhong 030619, China Key Laboratory of Cellular Physiology (Ministry of Education), Shanxi Medical University Taiyuan 030032, China Neurosurgery Department of Sinopharm Tongmei General Hospital,Key Laboratory of Neurological Disease Prevention and Control of Health Commission of Shanxi Province Datong 037003, China.
- Publication Type:Journal Article
- Keywords:
Buyang Huanwu Decoction;
inflammation;
oxidative stress;
oxygen-glucose deprivation/reglucose-reoxygenation
- MeSH:
Oxidative Stress/drug effects*;
Drugs, Chinese Herbal/pharmacology*;
Animals;
Mice;
Glucose/metabolism*;
Cell Line;
Inflammation/genetics*;
Oxygen/metabolism*;
Pyrazines/pharmacology*;
Microglia/metabolism*;
NF-E2-Related Factor 2/immunology*;
NF-kappa B/immunology*;
Toll-Like Receptor 4/immunology*;
Anti-Inflammatory Agents/pharmacology*;
Humans
- From:
China Journal of Chinese Materia Medica
2025;50(14):3835-3846
- CountryChina
- Language:Chinese
-
Abstract:
This study aims to explore the effects and action mechanisms of the active ingredients in Buyang Huanwu Decoction(BYHWD), namely tetramethylpyrazine(TMP) and hydroxy-safflor yellow A(HSYA), on oxygen-glucose deprivation/reglucose-reoxygenation(OGD/R)-induced inflammation and oxidative stress of microglia(MG). Network pharmacology was used to screen the effective monomer ingredients of BYHWD and determine the safe concentration range for each component. Inflammation and oxidative stress models were established to further screen the best ingredient combination and optimal concentration ratio with the most effective anti-inflammatory and antioxidant effects. OGD/R BV2 cell models were constructed, and BV2 cells in the logarithmic growth phase were divided into a normal group, a model group, an HSYA group, a TMP group, and an HSYA + TMP group. Enzyme-linked immunosorbent assay(ELISA) was used to detect the levels of inflammatory cytokines such as interleukin-1β(IL-1β), tumor necrosis factor-α(TNF-α), and interleukin-6(IL-6). Oxidative stress markers, including superoxide dismutase(SOD), nitric oxide(NO), and malondialdehyde(MDA), were also measured. Western blot was used to analyze the protein expression of both inflammation-related pathway [Toll-like receptor 4(TLR4)/nuclear factor-kappa B(NF-κB)] and oxidative stress-related pathway [nuclear factor erythroid 2-related factor 2(Nrf2)/heme oxygenase-1(HO-1)]. Immunofluorescence was used to assess the expression of proteins such as inducible nitric oxide synthase(iNOS) and arginase-1(Arg-1). The most effective ingredients for anti-inflammatory and antioxidant effects in BYHWD were TMP and HSYA. Compared to the normal group, the model group showed significantly increased levels of IL-1β, TNF-α, IL-6, NO, and MDA, along with significantly higher protein expression of NF-κB, TLR4, Nrf2, and HO-1 and significantly lower SOD levels. The differences between the two groups were statistically significant. Compared to the model group, both the HSYA group and the TMP group showed significantly reduced levels of IL-1β, TNF-α, IL-6, NO, and MDA, lower expression of NF-κB and TLR4 proteins, higher levels of SOD, and significantly increased protein expression of Nrf2 and HO-1. Additionally, the expression of the M1-type MG marker iNOS was significantly reduced, while the expression of the M2-type MG marker Arg-1 was significantly increased. The results of the HSYA group and the TMP group had statistically significant differences from those of the model group. Compared to the HSYA group and the TMP group, the HSYA + TMP group showed further significant reductions in IL-1β, TNF-α, IL-6, NO, and MDA levels, along with significant reductions in NF-κB and TLR4 protein expression, an increase in SOD levels, and elevated Nrf2 and HO-1 protein expression. Additionally, the expression of the M1-type MG marker iNOS was reduced, while the M2-type MG marker Arg-1 expression increased significantly in the HSYA + TMP group compared to the TMP or HSYA group. The differences in the results were statistically significant between the HSYA + TMP group and the TMP or HSYA group. The findings indicated that the combined use of HSYA and TMP, the active ingredients of BYHWD, can effectively inhibit OGD/R-induced inflammation and oxidative stress of MG, showing superior effects compared to the individual use of either component.
