Characterization of hippocampal components of Danzhi Xiaoyao Formula based on HPLC-Q-TOF-MS/MS and network pharmacology and assessment of its therapeutic potential for nervous system diseases.
10.19540/j.cnki.cjcmm.20250426.201
- Author:
Wen-Qing HU
1
;
Hui-Yuan GAO
2
;
Li YANG
3
;
Yu-Xin WANG
3
;
Hao-Jie CHENG
3
;
Si-Yu YANG
4
;
Mei-Yu ZHANG
3
;
Jian SUN
3
Author Information
1. School of Traditional Chinese Materia Medica, Shenyang Pharmaceutical University Benxi 117004, China Experimental Research Center, China Academy of Chinese Medical Sciences Beijing 100700, China.
2. School of Traditional Chinese Materia Medica, Shenyang Pharmaceutical University Benxi 117004, China.
3. Experimental Research Center, China Academy of Chinese Medical Sciences Beijing 100700, China.
4. Experimental Research Center, China Academy of Chinese Medical Sciences Beijing 100700, China Heilongjiang University of Chinese Medicine Harbin 150040, China.
- Publication Type:Journal Article
- Keywords:
Danzhi Xiaoyao Formula;
HPLC-Q-TOF-MS/MS;
hippocampal components;
network pharmacology;
therapeutic potential
- MeSH:
Drugs, Chinese Herbal/administration & dosage*;
Animals;
Rats;
Hippocampus/metabolism*;
Network Pharmacology;
Chromatography, High Pressure Liquid;
Tandem Mass Spectrometry;
Rats, Sprague-Dawley;
Male;
Nervous System Diseases/genetics*;
Humans;
Signal Transduction/drug effects*
- From:
China Journal of Chinese Materia Medica
2025;50(14):4053-4062
- CountryChina
- Language:Chinese
-
Abstract:
In this study, the pharmacodynamic components and potential pharmacological functions of Danzhi Xiaoyao Formula in treating nervous system diseases were investigated by hippocampal component characterization and network pharmacology. After rats were administrated with Danzhi Xiaoyao Formula by gavage, high performance liquid chromatography coupled with quadrupole time-of-flight tandem mass spectrometry(HPLC-Q-TOF-MS/MS) was employed to explore the components in the hippocampus of rats. Fifty-seven components were identified in the hippocampus of rats by comparing the extract of Danzhi Xiaoyao Formula, herbal components in the hippocampus after administration, and blank samples. KEGG and GO analyses predicted 74 core targets including GSK3B, MAPK1, AKT, IL6. These targets were involved in PI3K/Akt, NF-κB, MAPK, JAK/STAT, Wnt, and other signaling pathways. The results indicated that Danzhi Xiaoyao Formula may ameliorate other nervous system diseases enriched in DO, such as neurodegenerative diseases, cerebrovascular diseases, and mental and emotional disorders by mediating target pathways, inhibiting inflammation, reducing neuronal damage, and alleviating hippocampal atrophy. The relevant activities exhibited by this formula in nervous system diseases such as Alzheimer's disease, Parkinson's disease, and diabetic neuropathy have extremely high development value and are worthy of further in-depth research. This study provides a theoretical basis and practical guidance for expanding the application of Danzhi Xiaoyao Formula in the treatment of nervous system diseases.
