Effect and mechanism of Xintong Granules in ameliorating myocardial ischemia-reperfusion injury in rats by regulating gut microbiota.
10.19540/j.cnki.cjcmm.20250311.705
- Author:
Yun-Jia WANG
1
;
Ji-Dong ZHOU
2
;
Qiu-Yu SU
1
;
Jing-Chun YAO
2
;
Rui-Qiang SU
2
;
Guo-Fei QIN
2
;
Gui-Min ZHANG
2
;
Hong-Bao LIANG
3
;
Shuai FENG
1
;
Jia-Cheng ZHANG
4
Author Information
1. College of Pharmacy, Shandong University of Traditional Chinese Medicine Ji'nan 250355, China.
2. National Key Laboratory of Integration and Innovation of Jing Fang and Modern Chinese Medicine, Lunan Pharmaceutical Group Co., Ltd. Linyi 276006, China.
3. Shandong College of Traditional Chinese Medicine Yantai 264199, China.
4. Affiliated Hospital of Shandong University of Traditional Chinese Medicine Ji'nan 250355, China.
- Publication Type:Journal Article
- Keywords:
Xintong Granules;
efficacy evaluation;
gut microbiota;
myocardial ischemia-reperfusion injury;
short-chain fatty acids
- MeSH:
Animals;
Gastrointestinal Microbiome/drug effects*;
Rats;
Male;
Myocardial Reperfusion Injury/genetics*;
Drugs, Chinese Herbal/administration & dosage*;
Rats, Sprague-Dawley;
Apoptosis/drug effects*;
Humans;
Tumor Necrosis Factor-alpha/metabolism*;
Interleukin-6/genetics*;
Malondialdehyde/metabolism*
- From:
China Journal of Chinese Materia Medica
2025;50(14):4003-4014
- CountryChina
- Language:Chinese
-
Abstract:
This study investigates the mechanism by which Xintong Granules improve myocardial ischemia-reperfusion injury(MIRI) through the regulation of gut microbiota and their metabolites, specifically short-chain fatty acids(SCFAs). Rats were randomly divided based on body weight into the sham operation group, model group, low-dose Xintong Granules group(1.43 g·kg~(-1)·d~(-1)), medium-dose Xintong Granules group(2.86 g·kg~(-1)·d~(-1)), high-dose Xintong Granules group(5.72 g·kg~(-1)·d~(-1)), and metoprolol group(10 mg·kg~(-1)·d~(-1)). After 14 days of pre-administration, the MIRI rat model was established by ligating the left anterior descending coronary artery. The myocardial infarction area was assessed using the 2,3,5-triphenyltetrazolium chloride(TTC) staining method. Apoptosis in tissue cells was detected by the terminal deoxynucleotidyl transferase-mediated dUTP nick-end labeling(TUNEL) assay. Pathological changes in myocardial cells and colonic tissue were observed using hematoxylin-eosin(HE) staining. The levels of tumor necrosis factor-α(TNF-α), interleukin-1β(IL-1β), interleukin-6(IL-6), creatine kinase MB isoenzyme(CK-MB), and cardiac troponin T(cTnT) in rat serum were quantitatively measured using enzyme-linked immunosorbent assay(ELISA) kits. The activities of lactate dehydrogenase(LDH), creatine kinase(CK), and superoxide dismutase(SOD) in myocardial tissue, as well as the level of malondialdehyde(MDA), were determined using colorimetric assays. Gut microbiota composition was analyzed by 16S rDNA sequencing, and fecal SCFAs were quantified using gas chromatography-mass spectrometry(GC-MS). The results show that Xintong Granules significantly reduced the myocardial infarction area, suppressed cardiomyocyte apoptosis, and decreased serum levels of pro-inflammatory cytokines(TNF-α, IL-1β, and IL-6), myocardial injury markers(CK-MB, cTnT, LDH, and CK), and oxidative stress marker MDA. Additionally, Xintong Granules significantly improved intestinal inflammation in MIRI rats, regulated gut microbiota composition and diversity, and increased the levels of SCFAs(acetate, propionate, isobutyrate, etc.). In summary, Xintong Granules effectively alleviate MIRI symptoms. This study preliminarily confirms that Xintong Granules exert their inhibitory effects on MIRI by regulating gut microbiota imbalance and increasing SCFA levels.
