Effect of Modified Yiyi Fuzi Baijiang Powder on intestinal mucosal permeability and expression of AQP3, AQP4 in ulcerative colitis rats.
10.19540/j.cnki.cjcmm.20250311.401
- Author:
Wen-Xiao LI
1
;
Jiang CHEN
2
;
Zhi-Cheng HE
1
;
Lu-Rong ZHANG
3
;
Guo-Qiang LIANG
3
;
Xing-Xing JIANG
4
;
Yong-Na WEI
4
;
Qin ZHOU
4
Author Information
1. Shanghai University of Traditional Chinese Medicine Shanghai 201203, China.
2. Suzhou Hospital of Traditional Chinese Medicine, Shanghai University of Traditional Chinese Medicine Suzhou 215009, China.
3. Suzhou Academy of Wumen Chinese Medicine, Suzhou Hospital of Traditional Chinese Medicine Suzhou 215009, China.
4. Nanjing University of Chinese Medicine Nanjing 210023, China.
- Publication Type:Journal Article
- Keywords:
Modified Yiyi Fuzi Baijiang Powder;
aquaporins;
intestinal mucosal permeability;
tight junction;
ulcerative colitis
- MeSH:
Animals;
Colitis, Ulcerative/genetics*;
Drugs, Chinese Herbal/administration & dosage*;
Rats, Sprague-Dawley;
Rats;
Intestinal Mucosa/metabolism*;
Male;
Aquaporin 3/metabolism*;
Aquaporin 4/metabolism*;
Permeability/drug effects*;
Humans;
Powders;
Intestinal Barrier Function
- From:
China Journal of Chinese Materia Medica
2025;50(14):3962-3968
- CountryChina
- Language:Chinese
-
Abstract:
This study investigated the therapeutic effects and mechanisms of Modified Yiyi Fuzi Baijiang Powder on ulcerative colitis(UC) in rats from the perspective of dampness. SD rats were randomly allocated into six groups(n=10): control, model, mesalazine, and Modified Yiyi Fuzi Baijiang Powder at low(3.96 g·kg~(-1)·d~(-1)), medium(7.92 g·kg~(-1)·d~(-1)), and high(15.84 g·kg~(-1)·d~(-1)) doses. UC was induced in all groups except the control by administration with 3% dextran sulfate sodium(DSS) solution for 7 days. The disease activity index(DAI) was recorded, and the colon tissue was collected for analysis. Histopathological changes were assessed by hematoxylin-eosin staining. Serum levels of D-lactic acid(D-LA) and diamine oxidase(DAO) were measured by ELISA. Immunohistochemistry and PCR were employed to evaluate the expression of aquaporins(AQP3, AQP4) and tight junction proteins [zonula occludens-1(ZO-1) and occludin] at both protein and mRNA levels. Compared with the control group, the model group showed an increased DAI scores(P<0.05), intestinal mucosal damage, elevated serum levels of DAO and D-LA(P<0.05), and decreased expression of AQP3, AQP4, ZO-1, and occludin(P<0.05). Treatment with Modified Yiyi Fuzi Baijiang Powder reduced the DAI scores(P<0.05), lowered the serum levels of D-LA and DAO(P<0.05), and upregulated the expression of AQP3, AQP4, ZO-1, and occludin at both protein and mRNA levels compared with the model group. These findings suggest that Modified Yiyi Fuzi Baijiang Powder exerts therapeutic effects on UC by reducing the intestinal mucosal permeability, promoting colonic mucosal repair, and regulating abnormal intestinal water metabolism, which may involve the upregulation of AQP3 and AQP4 expression.
