Quality evaluation of Bidentis Herba based on HPLC fingerprint, multi-component content determination, and chemometrics.
10.19540/j.cnki.cjcmm.20250417.201
- Author:
Guo-Li SHI
1
;
Xin-Feng WANG
2
;
Wei-Qun LI
3
;
Jian-Wei FAN
3
;
Yong-Xia GUAN
4
Author Information
1. Shandong University of Traditional Chinese Medicine Ji'nan 250355, China.
2. Affiliated Hospital of Shandong University of Traditional Chinese Medicine Ji'nan 250014, China.
3. State Key Laboratory of Integration and Innovation of Classic Formula and Modern Chinese Medicine,Lunan Pharmaceutical Group Co., Ltd. Linyi 276005, China.
4. Shandong University of Traditional Chinese Medicine Ji'nan 250355, China State Key Laboratory of Integration and Innovation of Classic Formula and Modern Chinese Medicine,Lunan Pharmaceutical Group Co., Ltd. Linyi 276005, China.
- Publication Type:English Abstract
- Keywords:
B. pilosa;
Bidens pilosa var. radiata;
cluster analysis;
content determination;
fingerprint;
principal component analysis;
quality control
- MeSH:
Chromatography, High Pressure Liquid/methods*;
Drugs, Chinese Herbal/chemistry*;
Quality Control;
Chemometrics/methods*;
Bidens/chemistry*;
Principal Component Analysis
- From:
China Journal of Chinese Materia Medica
2025;50(14):3944-3950
- CountryChina
- Language:Chinese
-
Abstract:
This study established the HPLC fingerprints and a multi-component content determination method for Bidens pilosa var. radiata and B. pilosa and conducted comprehensive evaluation by integrating fingerprint similarity comparison, cluster analysis(CA), and principal component analysis(PCA), aiming to provide a reference for the establishment of quality standards for Bidentis Herba. HPLC was launched on an Agilent Poroshell 120 EC-C_(18) chromatographic column(4.6 mm×250 mm, 4 μm) by gradient elution with a mobile phase of 0.1% aqueous phosphoric acid-acetonitrile at a flow rate of 0.7 mL·min~(-1), detection wavelength of 270 nm, column temperature of 25 ℃, and an injection volume of 5 μL. The fingerprint similarity of 20 batches of Bidentis Herba ranged from 0.775 to 0.979. A total of 20 common peaks were identified, and seven components were confirmed through comparison with reference substances: neochlorogenic acid, chlorogenic acid, isochlorogenic acid A, isochlorogenic acid B, isochlorogenic acid C, rutin, and hyperoside. These seven components exhibited good linearity within the ranges of 3.4-67.4, 33.0-660.3, 26.6-531.2, 3.5-70.5, 6.2-124.9, 2.4-48.3, and 4.6-91.5 μg·mL~(-1), respectively, with correlation coefficients(r) greater than 0.999. The average recovery rates ranged from 96.47% to 104.6%. CA and PCA classified the 20 batches of Bidentis Herba into two categories. PCA yielded two principal components, with a cumulative variance contribution rate of 80.557%. The established HPLC fingerprints and multi-component content determination method are simple and accurate, providing a scientific basis for the quality control and quality standard formulation of Bidentis Herba.
