Identification of critical quality attributes related to property and flavor of Jianwei Xiaoshi Tablets based on T1R2/T1R3/TRPV1-HEMT biosensor.
10.19540/j.cnki.cjcmm.20250321.304
- Author:
Dong-Hong LIU
1
;
Yan-Yu HAN
2
;
Jing WANG
2
;
Hai-Yang LI
2
;
Xin-Yu GUO
2
;
Hui-Min FENG
2
;
Han HE
2
;
Shuo-Shuo XU
2
;
Zhi-Jian ZHONG
3
;
Zhi-Sheng WU
1
Author Information
1. School of Pharmacy,Fujian University of Traditional Chinese Medicine Fuzhou 350122,China School of Chinese Materia Medica, Beijing University of Chinese Medicine Beijing 102488, China Engineering Research Center of Chinese Medicine Production and New Drug Development, Ministry of Education Beijing 102488, China.
2. School of Chinese Materia Medica, Beijing University of Chinese Medicine Beijing 102488, China Engineering Research Center of Chinese Medicine Production and New Drug Development, Ministry of Education Beijing 102488, China.
3. Huarun Jiangzhong Pharmaceutical Group Co.,Ltd. Nanchang 330096, China.
- Publication Type:Journal Article
- Keywords:
Jianwei Xiaoshi Tablets;
biosensor;
chemical composition;
critical quality attributes;
traditional Chinese medicine manufacturing
- MeSH:
Drugs, Chinese Herbal/chemistry*;
Biosensing Techniques/methods*;
TRPV Cation Channels/chemistry*;
Tablets/chemistry*;
Receptors, G-Protein-Coupled/genetics*;
Quality Control;
Taste;
Humans;
Mass Spectrometry
- From:
China Journal of Chinese Materia Medica
2025;50(14):3930-3937
- CountryChina
- Language:Chinese
-
Abstract:
The quality of traditional Chinese medicine(TCM) is a critical foundation for ensuring the stability of its efficacy, as well as the safety and effectiveness of its clinical use. The identification of critical quality attributes(CQAs) is one of the core components of TCM preparation quality control. This study focuses on Jianwei Xiaoshi Tablets and explores their CQAs related to property and flavor from the perspective of taste receptor proteins. Three taste receptor proteins, T1R2, T1R3, and TRPV1, were selected, and a biosensor based on high-electron-mobility transistor(HEMT) was constructed to detect the interactions between Jianwei Xiaoshi Tablets and taste receptor proteins. Simultaneously, liquid chromatography-mass spectrometry(LC-MS) technology was used to analyze the chemical composition of Jianwei Xiaoshi Tablets. In examining the interaction strength, the results indicated that the interaction between Jianwei Xiaoshi Tablets and TRPV1 protein was the strongest, followed by T1R3, with the interaction with T1R2 being relatively weaker. By combining biosensing technology with LC-MS, 16 chemical components were identified from Jianwei Xiaoshi Tablets, among which six were selected as CQAs for sweetness and seven for pungency. Further validation experiments demonstrated that CQAs such as hesperidin and hesperetin had strong interactions with their corresponding taste receptor proteins. Through the combined use of multiple technological approaches, this study successfully determined the property and flavor-related CQAs of Jianwei Xiaoshi Tablets. It provides novel ideas and approach for the identification of CQAs in TCM preparations and offers comprehensive theoretical support for TCM quality control, contributing to the improvement and development of TCM preparation quality control systems.
