Effect of different phosphorus application on morphological traits, active ingredients and rhizosphere soil microbial community of Polygala tenuifolia.
10.19540/j.cnki.cjcmm.20250308.101
- Author:
Huan GUO
1
;
Tong WEI
1
;
Wen-Hua CUI
1
;
Huan SHI
1
;
Fu-Ying MAO
2
;
Xian GU
1
;
Yun-Sheng ZHAO
3
;
Xiao-Feng LIANG
4
Author Information
1. College of Pharmacy, Hebei University of Chinese Medicine Shijiazhuang 050200, China Traditional Chinese Medicine Processing Technology Innovation Center of Hebei Province,Hebei University of Chinese Medicine Shijiazhuang 050091, China.
2. Experimental Center, Hebei University of Chinese Medicine Shijiazhuang 050200, China.
3. College of Pharmacy, Hebei University of Chinese Medicine Shijiazhuang 050200, China Traditional Chinese Medicine Processing Technology Innovation Center of Hebei Province,Hebei University of Chinese Medicine Shijiazhuang 050091, China International Joint Research Center on Resource Utilization and Quality Evaluation of Traditional Chinese Medicine of Hebei Province Shijiazhuang 050091, China.
4. Mianyang Key Laboratory of Development and Utilization of Chinese Medicine Resources, Sichuan College of Traditional Chinese Medicine Mianyang 621000, China.
- Publication Type:Journal Article
- Keywords:
Polygala tenuifolia;
active ingredients;
morphological traits;
phosphorus fertilizer;
soil microorganisms
- MeSH:
Rhizosphere;
Phosphorus/pharmacology*;
Soil Microbiology;
Polygala/anatomy & histology*;
Fertilizers/analysis*;
Bacteria/metabolism*;
Soil/chemistry*;
Microbiota/drug effects*;
Plant Roots/metabolism*
- From:
China Journal of Chinese Materia Medica
2025;50(14):3898-3908
- CountryChina
- Language:Chinese
-
Abstract:
To investigate the effects of phosphorus fertilizer on the morphological traits, active ingredients and rhizosphere soil microbial community of Polygala tenuifolia. The phosphorus fertilizer was calculated in terms of P_2O_5. Five treatments were set up: 0(CK), 17(P1), 34(P2), 51(P3), and 68(P4) kg per Mu(1 Mu≈667 m~2). A randomized block design was adopted. Samples of P. tenuifolia and its rhizosphere soil were collected under different superphosphate fertilizer treatments. Illumina high-throughput sequencing was used to analyze the rhizosphere soil microbial community, 9 morphological traits were measured and the content of 11 active ingredients were determined. The results showed that the whole plant weight, shoot fresh weight, root weight, and root peel thickness were the highest under P1 treatment, increasing by 34.41%, 38.80%, 39.21%, and 3.17% respectively compared to CK. Under P2 treatment, the plant height, stem diameter, root thickness, and core thickness were significantly higher than CK. Phosphorus fertilizer had a significant impact on the content of tenuifolin, sibiricose A5, sibiricose A6, arillanin A, 3,6'-disinapoyl sucrose, and polygalaxanthone Ⅲ. Correlation analysis results showed that the relative abundance of Arthrobacter, Bacillus, norank＿f＿Vicinamibacteraceae, norank＿o＿Vicinamibacterales, MND1 and other bacteria, as well as the relative abundance of Neocosmospora, Paraphoma and other fungi were positively correlated with root diameter, wood core diameter, the whole plant weight, root weight, shoot fresh weight of P. tenuifolia. Bacillus, Neocosmospora, Subulicystidium were significantly positively correlated with oligosaccharides such as 3,6'-disinapoyl sucrose, sibiricose A5、sibiricose A6、glomeratose A、arillanin A and tenuifoliside C. Arthrobacter, Humicola, Aspergillus, Paraphoma were positively correlated with tenuifolin and norank＿f＿Vicinamibacteraceae, norank＿o＿Vicinamibacterales, Fusarium were positively correlated with polygalaxanthone Ⅲ. Evidently, appropriate phosphorus application is conducive to the growth and quality improvement of P. tenuifolia, and can increase the abundance of beneficial microorganisms in the soil.
