Optimal harvesting period of cultivated Notopterygium incisum based on HPLC specific chromatogram combined with chemometrics and entropy weight-gray correlation analysis.
10.19540/j.cnki.cjcmm.20250416.102
- Author:
Jing-Cheng WANG
1
;
Hong-Bing SUN
2
;
Teng LIU
2
;
Wen-Tao ZHU
2
;
Hong-Lan WANG
2
;
Yi ZHOU
2
;
Wei-Yan WANG
1
;
Ping YANG
2
;
Shun-Yuan JIANG
1
Author Information
1. School of Pharmacy, Chengdu University of Traditional Chinese Medicine Chengdu 611137, China Sichuan Academy of Chinese Medicine Sciences Chengdu 610041, China.
2. Sichuan Academy of Chinese Medicine Sciences Chengdu 610041, China.
- Publication Type:Evaluation Study
- Keywords:
cultivated Notopterygium incisum;
entropy weight-gray correlation analysis;
harvesting period;
quality evaluation;
specific chromatogram
- MeSH:
Chromatography, High Pressure Liquid/methods*;
Apiaceae/chemistry*;
Entropy;
Chemometrics/methods*;
Drugs, Chinese Herbal/chemistry*;
Principal Component Analysis;
Quality Control
- From:
China Journal of Chinese Materia Medica
2025;50(14):3878-3886
- CountryChina
- Language:Chinese
-
Abstract:
To determine the optimal cultivation duration and harvest period for cultivated Notopterygium incisum and promote its industrial development, this study established a characteristic chromatographic profile of cultivated N. incisum and employed chemometrics combined with entropy-weighted grey correlation analysis to assess differences in agronomic traits and quality indicators across different cultivation years and harvest periods. By comparing with reference substances, ten common peaks were identified, including chlorogenic acid, p-coumaric acid, ferulic acid, marmesinin, nodakenin, isochlorogenic acid B, notopterol, phenethyl ferulate, isoimperatorin, and falcarindiol. The similarity between the characteristic chromatographic profiles of N. incisum at different cultivation years and the reference profile was all above 0.932. Principal component analysis(PCA) and orthogonal partial least squares discriminant analysis(OPLS-DA) revealed that the quality of 1-to 3-year-old cultivated N. incisum was highly dispersed and unstable, whereas the quality of 4-year-old cultivated N. incisum remained relatively stable across different harvest periods. This suggests that the accumulation of relevant compounds in the medicinal material had reached a plateau, confirming that the optimal cultivation period for N. incisum is four years. Entropy-weighted grey correlation analysis indicated that the quality of 4-year-old cultivated N. incisum across different harvest periods ranked from highest to lowest as follows: November, December, October, August, July, and September, demonstrating that November is the optimal harvest time. The findings of this study establish the suitable cultivation duration and optimal harvest period for N. incisum, providing a scientific basis for cultivation guidance and quality standardization.
