Rapid characterization and identification of non-volatile components in Rhododendron tomentosum by UHPLC-Q-TOF-MS method.
10.19540/j.cnki.cjcmm.20250203.201
- Author:
Su-Ping XIAO
1
;
Long-Mei LI
2
;
Bin XIE
2
;
Hong LIANG
3
;
Qiong YIN
4
;
Jian-Hui LI
5
;
Jie DU
6
;
Ji-Yong WANG
6
;
Run-Huai ZHAO
6
;
Yan-Qin XU
4
;
Yun-Bo SUN
7
;
Zong-Yuan LU
8
;
Peng-Fei TU
3
Author Information
1. School of Pharmaceutical Sciences, Peking University Beijing 100871, China China National Traditional Chinese Medicine Co., Ltd. Beijing 100195, China.
2. China National Traditional Chinese Medicine Co., Ltd. Beijing 100195, China Jiangxi University of Traditional Chinese Medicine Nanchang 330004, China.
3. School of Pharmaceutical Sciences, Peking University Beijing 100871, China.
4. Jiangxi University of Traditional Chinese Medicine Nanchang 330004, China.
5. Agriculture and Rural Bureau of the Administrative Office of Daxing'anling Region,Heilongjiang Province Jiagedaqi 165000, China.
6. China National Traditional Chinese Medicine Co., Ltd. Beijing 100195, China.
7. Sinopharm Group Beijing HuaMiao Pharmaceutical Co., Ltd. Beijing 102600, China.
8. Shanghai Nature Standard Technology Co., Ltd. Shanghai 201314, China.
- Publication Type:English Abstract
- Keywords:
Rhododendron tomentosum;
UHPLC-Q-TOF-MS;
flavonoid;
flavonoid glycoside;
fragmentation pattern;
phenolic glycoside
- MeSH:
Rhododendron/chemistry*;
Chromatography, High Pressure Liquid/methods*;
Drugs, Chinese Herbal/chemistry*;
Mass Spectrometry/methods*;
Plant Leaves/chemistry*
- From:
China Journal of Chinese Materia Medica
2025;50(11):3054-3069
- CountryChina
- Language:Chinese
-
Abstract:
This study aimed to characterize and identify the non-volatile components in aqueous and ethanolic extracts of the stems and leaves of Rhododendron tomentosum by using sensitive and efficient ultra-performance liquid chromatography-quadrupole-time of flight mass spectrometry(UHPLC-Q-TOF-MS) combined with a self-built information database. By comparing with reference compounds, analyzing fragment ion information, searching relevant literature, and using a self-built information database, 118 compounds were identified from the aqueous and ethanolic extracts of R. tomentosum, including 35 flavonoid glycosides, 15 phenolic glycosides, 12 flavonoids, 7 phenolic acids, 7 phenylethanol glycosides, 6 tannins, 6 phospholipids, 5 coumarins, 5 monoterpene glycosides, 6 triterpenes, 3 fatty acids, and 11 other types of compounds. Among them, 102 compounds were reported in R. tomentosum for the first time, and 36 compounds were identified by comparing them with reference compounds. The chemical components in the ethanolic and aqueous extracts of R. tomentosum leaves and stems showed slight differences, with 84 common chemical components accounting for 71.2% of the total 118 compounds. This study systematically characterized and identified the non-volatile chemical components in the ethanolic and aqueous extracts of R. tomentosum for the first time. The findings provide a reference for active ingredient research, quality control, and product development of R. tomentosum.
