Establishment of tissue culture and rapid propagation system of Artemisia stolonifera.
10.19540/j.cnki.cjcmm.20241215.101
- Author:
Chu WANG
1
;
Ya XU
1
;
Yang XU
1
;
Ye WANG
1
;
Na-Na CHANG
1
;
Lu-Qi HUANG
2
;
Hui LI
3
Author Information
1. Jiangxi Key Laboratory for Sustainable Utilization of Chinese Materia Medica Resources, Institute of Traditional Chinese Medicine Health Industry, China Academy of Chinese Medical Sciences Nanchang 330115, China Jiangxi Institute of Traditional Chinese Medicine Health Industry Nanchang 330115, China.
2. China Academy of Chinese Medical Sciences Beijing 100700, China.
3. Jiangxi Key Laboratory for Sustainable Utilization of Chinese Materia Medica Resources, Institute of Traditional Chinese Medicine Health Industry, China Academy of Chinese Medical Sciences Nanchang 330115, China Jiangxi Institute of Traditional Chinese Medicine Health Industry Nanchang 330115, China Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences Beijing 100700, China.
- Publication Type:English Abstract
- Keywords:
Artemisia stolonifera;
acclimatization and transplanting;
in vitro rapid propagation;
multiplication;
rooting
- MeSH:
Artemisia/drug effects*;
Tissue Culture Techniques/methods*;
Plant Growth Regulators/pharmacology*;
Plant Stems/drug effects*;
Plant Shoots/drug effects*
- From:
China Journal of Chinese Materia Medica
2025;50(11):2994-3000
- CountryChina
- Language:Chinese
-
Abstract:
As a high-quality moxibustion material, Artemisia stolonifera has high economic value and research prospects. However, due to difficulties in seed germination, its wild germplasm resources are sparsely distributed in China. This study used young stem segments grown in the current year to investigate the effects of explant sterilization, different combinations and concentrations of plant growth regulators on the proliferation and rooting of adventitious shoots, with the aim of constructing an in vitro rapid propagation technology system for A. stolonifera. The results showed that the lowest contamination rate of 25.83% was achieved when sterilizing the stem segments by rinsing with running water for 30 min, soaking in 75% ethanol for 30 s, followed by a 5 min treatment with 0.1% HgCl_2, 10 min with 8% NaClO, and 10 min with 0.6% phytosaniline. There was no browning of the stem segments, and surface sterilization of the A. stolonifera stem segments was successfully achieved. In the induction culture phase, when the concentration of kinetin(KT) was 0.05 mg·L~(-1) and 6-benzylaminopurine(6-BA) was 0.05 mg·L~(-1), the adventitious shoot proliferation coefficient was 2.02, effectively promoting the proliferation and growth of A. stolonifera. In the rooting culture phase, 0.1 mg·L~(-1) 1-naphthaleneacetic acid(NAA) effectively induced A. stolonifera test-tube seedlings to root within a short period, achieving a rooting rate of 100%. The addition of a small amount of activated charcoal also promoted rooting and strengthened seedling growth. The survival rate of A. stolonifera seedlings transplanted into a substrate consisting of 90% nutrient soil and 10% perlite was 100%. This study established an efficient in vitro rapid propagation system for A. stolonifera, overcoming difficulties with seed germination, shortening the breeding cycle, and reducing production and planting costs. It provides technical support for the introduction, domestication, seedling propagation, germplasm conservation, and industrial development of A. stolonifera.
