Protective effect of ethyl syringate against ulcerative colitis based on JAK2/STAT3 pathway.
10.19540/j.cnki.cjcmm.20250103.705
- Author:
Meng-di LIANG
1
;
Yue-Run LIANG
1
;
Jin CHENG
1
;
Ya-Ping YANG
1
;
Xuan XIA
2
;
Wen-Zhe YANG
3
;
Jie-Jie HAO
1
Author Information
1. Ministry of Education Key Laboratory of Marine Drugs,School of Medicine and Pharmacy, Ocean University of China Qingdao 266005,China.
2. Marine Biomedical Research Institute of Qingdao Qingdao 266100, China.
3. Ministry of Education Key Laboratory of Marine Drugs,School of Medicine and Pharmacy, Ocean University of China Qingdao 266005,China Marine Biomedical Research Institute of Qingdao Qingdao 266100, China.
- Publication Type:Journal Article
- Keywords:
JAK2/STAT pathway;
ethyl syringate;
intestinal barrier function;
intestinal inflammation;
ulcerative colitis
- MeSH:
Animals;
Colitis, Ulcerative/chemically induced*;
Janus Kinase 2/genetics*;
STAT3 Transcription Factor/genetics*;
Mice;
Humans;
Signal Transduction/drug effects*;
Male;
RAW 264.7 Cells;
Reactive Oxygen Species/metabolism*;
Nitric Oxide/metabolism*;
HT29 Cells;
Salicylates/administration & dosage*;
Protective Agents/administration & dosage*
- From:
China Journal of Chinese Materia Medica
2025;50(10):2778-2786
- CountryChina
- Language:Chinese
-
Abstract:
To study the therapeutic effect and mechanisms of ethyl syringate(MD) on ulcerative colitis(UC), the MTT assay was used to detect the proliferation inhibition of RAW264.7 cells and HT-29 cells by different concentrations of MD(50, 100, 200, 400 μmol·L~(-1)). UC cell models were constructed by inducing RAW264.7 cells and HT-29 cells with lipopolysaccharide(LPS) and tumor necrosis factor-α(TNF-α). An animal model was established by inducing mice with 2.5% dextran sulfate sodium(DSS) to verify the therapeutic effect of MD on UC. A control group, a model group(LPS or TNF-α), and groups treated with different concentrations of MD(50, 100, 200, 400 μmol·L~(-1)) were set up in this study. Nitric oxide(NO) levels were measured using a NO detection kit. Intracellular reactive oxygen species(ROS) levels were assessed using a laser confocal microscope and ROS kit. Enzyme-linked immunosorbent assay(ELISA) was used to detect changes in the levels of interleukin-6(IL-6), TNF-α, interferon-γ(INF-γ), interleukin-10(IL-10), and myeloperoxidase(MPO) in cells and animal tissues. Western blot was used to detect the expression levels of phosphorylated Janus kinase 2(p-JAK2), Janus kinase 2(JAK2), phosphorylated signal transducer and activator of transcription 3(p-STAT3), signal transducer and activator of transcription 3(STAT3), zonula occludens-1(ZO-1), occludin, and claudin-1 in cells and animal tissues. The results showed that MD can improve the inflammatory response by inhibiting the production of NO and ROS and regulating the expression of inflammatory factors. It significantly reduced the disease activity index(DAI) in mice, improved the shortening of the colon, and repaired intestinal epithelial damage by inhibiting the activation of the JAK2/STAT3 pathway, thereby exerting anti-UC activity.
