Mechanism of immediate administration of Angong Niuhuang Pills in intervention of traumatic brain injury based on metabolomics and transcriptomics.
10.19540/j.cnki.cjcmm.20250103.703
- Author:
Xiao-Tong ZHU
1
;
Liang-Liang TIAN
2
;
Jing-Jing ZHANG
3
;
Hong-Jun YANG
4
Author Information
1. Institute of Chinese Medicinal Sciences,Guangdong Pharmaceutical University Guangzhou 510006,China Institute of Chinese Materia Medica,China Academy of Chinese Medical Sciences Beijing 100700,China.
2. Institute of Chinese Materia Medica,China Academy of Chinese Medical Sciences Beijing 100700,China Jiangxi Province Key Laboratory of Traditional Chinese Medicine Pharmacology,Institute of Traditional Chinese Medicine Health Industry,China Academy of Chinese Medical Sciences Nanchang 330115,China.
3. Institute of Chinese Materia Medica,China Academy of Chinese Medical Sciences Beijing 100700,China Chinese Institute for Brain Research Beijing 102206,China.
4. Institute of Chinese Medicinal Sciences,Guangdong Pharmaceutical University Guangzhou 510006,China Beijing Key Laboratory of Traditional Chinese Medicine Basic Research on Prevention and Treatment for Major Diseases,Experimental Research Center,China Academy of Chinese Medical Sciences Beijing 100700,China.
- Publication Type:Journal Article
- Keywords:
Angong Niuhuang Pills;
glycerophospholipid metabolism;
metabolomics;
transcriptomics;
traumatic brain injury
- MeSH:
Animals;
Drugs, Chinese Herbal/administration & dosage*;
Rats;
Brain Injuries, Traumatic/metabolism*;
Male;
Metabolomics;
Rats, Sprague-Dawley;
Transcriptome/drug effects*;
Cyclooxygenase 2/genetics*;
Brain/metabolism*;
Caspase 3/genetics*;
Humans;
Tumor Suppressor Protein p53/genetics*
- From:
China Journal of Chinese Materia Medica
2025;50(10):2750-2760
- CountryChina
- Language:Chinese
-
Abstract:
This study integrates metabolomics and transcriptomics to explore the immediate effects of Angong Niuhuang Pills(ANP) in intervening traumatic brain injury(TBI) in rats. A TBI model was successfully established in rats using the optimized Feeney free-fall impact technique. Rats were randomly divided into sham operation(sham) group, model(Mod) group, positive drug(piracetam) group, ANP low-dose(ANP-L) group, and ANP high-dose(ANP-H) group according to a random number table. Nissl staining and immunofluorescence were used to count the number of Nissl bodies and detect B-cell lymphoma-2(Bcl-2) gene, caspase-3, and tumor protein 53(TP53) expression in brain tissue, and enzyme-linked immunosorbent assay(ELISA) was used to measure prostaglandin-endoperoxide synthase 2(PTGS2) level in rat brain tissue. Metabolomics and transcriptomics analyses were conducted for brain tissue from sham, Mod, and ANP-H groups. Gene Ontology(GO) and Kyoto Encyclopedia of Genes and Genomes(KEGG) enrichment analyses were carried out to indicate the mechanisms of ANP in the intervention of TBI. Integrative metabolomics and transcriptomics analysis revealed the metabolic pathways involved in ANP's intervention in TBI. The results showed that ANP significantly increased the number of Nissl bodies in TBI rat brain tissue, upregulated Bcl-2 expression, and downregulated the levels of caspase-3, TP53, and PTGS2. Compared to the Mod group, the ANP-H group significantly upregulated 12 differential metabolites(DMs) and downregulated 25 DMs. Five key metabolic pathways were identified, including glycerophospholipid metabolism, pyrimidine metabolism, glycine, threonine, and serine metabolism, arginine and proline metabolism, and D-amino acid metabolism. Transcriptomics identified 730 upregulated and 612 downregulated differentially expressed genes(DEGs). Enrichment analysis highlighted that biological functions related to inflammatory responses and apoptotic processes, and key signaling pathways, including phosphoinositide 3-kinase(PI3K)/protein kinase B(Akt) and mitogen-activated protein kinase(MAPK) were significantly enriched. The data of transcriptomics and metabolomics pinpointed three key metabolic pathways, i.e., glycerophospholipid metabolism, pyrimidine metabolism, and glycine, threonine, and serine metabolism.
