Two new lignans from Ajania purpurea.
10.19540/j.cnki.cjcmm.20250308.201
- Author:
Yu-Shun CUI
1
;
Min YAO
1
;
Xin-Jun DI
1
;
Zhi-Qiang LI
1
;
Shan HAN
2
;
Jun-Mao LI
2
;
Yu-Lin FENG
2
Author Information
1. Jiangxi University of Chinese Medicine Nanchang 330004, China.
2. Jiangxi University of Chinese Medicine Nanchang 330004, China National Pharmaceutical Engineering Center for Solid Preparation of Chinese Herbal Medicine Nanchang 330006, China.
- Publication Type:Journal Article
- Keywords:
Ajania purpurea;
anti-inflammatory;
ferroptosis;
lignan
- MeSH:
Mice;
Animals;
Lignans/isolation & purification*;
RAW 264.7 Cells;
Humans;
Nitric Oxide;
Tumor Necrosis Factor-alpha/immunology*;
Drugs, Chinese Herbal/isolation & purification*;
NF-E2-Related Factor 2/metabolism*;
Macrophages/metabolism*;
Interleukin-6/immunology*
- From:
China Journal of Chinese Materia Medica
2025;50(12):3322-3334
- CountryChina
- Language:Chinese
-
Abstract:
Macroporous resin adsorption column chromatography, silica gel column chromatography, ODS column chromatography, and semi-preparative high-performance liquid chromatography, combined with analytical methods such as NMR and MS, were employed to separate and identify compounds from the 70% ethanol extract of Ajania purpurea. A total of 30 compounds were isolated and identified, including 13 phenolic acids, 7 coumarins, 2 lignans, 1 flavonoid, 2 sesquiterpenes, 1 steroid, and 4 others. Among them, compounds 1 and 2 were newly discovered compounds, and compounds 4, 6, 8, 12, 14-23, 25, 28, and 30 were isolated from Ajania plants for the first time. Bioactivity screening showed that multiple compounds significantly inhibited the production of nitric oxide in lipopolysaccharide-stimulated RAW264.7 cells in a dose-dependent manner. Furthermore, compound 2 elevated the levels of glutathione in LPS-induced BEAS-2B cells, reduced the expression of pro-inflammatory cytokines such as tumor necrosis factor(TNF)-α, interleukin(IL)-6, and IL-1β, enhanced the mRNA of GPX4, HMOX1, NFE2L2, and enhanced protein levels of GPX4, HO-1, Nrf2, and SLC7A11, demonstrating potential anti-ferroptotic effect.
