Analysis of impact of host plants on quality of Taxilli Herba based on widely targeted metabolomics.
10.19540/j.cnki.cjcmm.20250314.101
- Author:
Dong-Lan ZHOU
1
;
Zi-Shu CHAI
2
;
Mei RU
3
;
Fei-Ying HUANG
3
;
Xie-Jun ZHANG
4
;
Min GUO
3
;
Yong-Hua LI
1
Author Information
1. College of Pharmacy, Guangxi University of Chinese Medicine Nanning 530200, China Guangxi Key Laboratory of Efficacy Study on Chinese Materia Medica Nanning 530200, China.
2. Faculty of Chinese Medicine Science, Guangxi University of Chinese Medicine Nanning 530200, China.
3. College of Pharmacy, Guangxi University of Chinese Medicine Nanning 530200, China.
4. Guangxi Wuzhou Shennong Liangpin Agricultural Technology Co., Ltd. Wuzhou 543213, China.
- Publication Type:Journal Article
- Keywords:
Taxilli Herba;
host;
quality impact;
widely targeted metabolomics
- MeSH:
Metabolomics/methods*;
Drugs, Chinese Herbal/chemistry*;
Chromatography, High Pressure Liquid;
Tandem Mass Spectrometry;
Quality Control;
Salix/chemistry*;
Cinnamomum aromaticum/metabolism*;
Principal Component Analysis
- From:
China Journal of Chinese Materia Medica
2025;50(12):3281-3290
- CountryChina
- Language:Chinese
-
Abstract:
This study aims to explore the impact of host plants on the quality of Taxilli Herba and provide a theoretical basis for the quality control of Taxilli Herba. The components of Taxilli Herba from three different host plants(Morus alba, Salix babylonica, and Cinnamomum cassia) and its 3 hosts(mulberry branch, willow branch, and cinnamon branch) were detected by widely targeted metabolomics based on ultra-high performance liquid chromatography-tandem mass spectrometry(UPLC-MS/MS). Principal component analysis(PCA), orthogonal partial least squares discriminant analysis(OPLS-DA), and Venn diagram were employed for analysis. A total of 717 metabolites were detected in Taxilli Herba from the three host plants and the branches of these host plants by UPLC-MS/MS. The results of PCA and OPLS-DA of Taxilli Herba from the three different host plants showed an obvious separation trend due to the different effects of host plants. The Venn diagram showed that there were 32, 8, and 26 characteristic metabolites in samples of Taxilli Herba from M. alba host, S. babylonica host, and C. cassia host, respectively. It was found by comparing the characteristic metabolites of Taxilli Herba and its hosts that each host transmits its characteristic components to Taxilli Herba, so that the Taxilli Herba contains the characteristic components of the host. The Kyoto Encyclopedia of Genes and Genomes(KEGG) pathway analysis showed that the differential metabolites of Taxilli Herba from the three hosts were mainly enriched in flavonoid biosynthesis, arginine and proline metabolism, and glycolysis/gluconeogenesis pathways. Furthermore, the differential metabolites enriching pathways of Taxilli Herba from the three hosts were different depending on the host. In a word, host plants have a significant impact on the metabolites of Taxilli Herba, and it may be an important factor for the quality of Taxilli Herba.
