Chemical constituents from Ecballium elaterium and their cytotoxicity.
10.19540/j.cnki.cjcmm.20250206.201
- Author:
Yu-Wei SUN
1
;
AYIZUOKELAMU YASEN
1
;
Xue-Rui AN
1
;
Wei LIU
1
;
Tao YUAN
2
Author Information
1. Xinjiang Key Laboratory of Clean Conversion and High Value Utilization of Biomass Resources,School of Chemistry and Chemical Engineering, Yili Normal University Yining 835000, China.
2. Xinjiang Key Laboratory of Clean Conversion and High Value Utilization of Biomass Resources,School of Chemistry and Chemical Engineering, Yili Normal University Yining 835000, China School of Health, Jiangxi Normal University Nanchang 330022, China.
- Publication Type:Journal Article
- Keywords:
Ecballium elaterium;
chemical constituents;
cucurbitacins;
cytotoxicity
- MeSH:
Humans;
Drugs, Chinese Herbal/toxicity*;
Cell Line, Tumor;
Cucurbitaceae/chemistry*;
Cell Survival/drug effects*
- From:
China Journal of Chinese Materia Medica
2025;50(8):2159-2166
- CountryChina
- Language:Chinese
-
Abstract:
To explore the chemical constituents of Ecballium elaterium and their cytotoxicity, this study employed multiple chromatographic techniques including normal-phase silica gel, MCI, octadecylsilyl(ODS), Sephadex LH-20 gel, and semi-preparative liquid chromatography for compound isolation from its active fraction. A total of 12 compounds were obtained, and they were identified according to the analysis of a variety of spectral data and literature comparison as 24Z-20,27-dihydroxy-16α,23α-epoxy-cucurbita-2-O-α-L-rhamnopyranosyl-(1→2)-β-D-glucopyranoside(1), cucurbitacin R(2), cucurbitacin B(3), cucurbitacin D(4), cucurbitacin I(5), cucurbitacin L(6), dehydrodiconiferyl alcohol(7), 3-hydroxy-4-methoxycinnamic acid(8), ferulaic acid(9), p-coumaric acid(10), rutin(11), and lariciresinol-4'-O-β-D-glucoside(12), among which compound 1 was a new compound. Compounds 2-6 had strong cytotoxicity against human lung carcinoma A549 cells with the IC_(50) values of(0.48±0.09),(0.03±0.002),(0.13±0.03),(0.87±0.14),(0.15±0.03) μmol·L~(-1), respectively, which were stronger than the positive control doxorubicin \[IC_(50)=(3.92±1.60) μmol·L~(-1)\].
