Intraspecific variation of Forsythia suspensa chloroplast genome.
10.19540/j.cnki.cjcmm.20250114.103
- Author:
Yu-Han LI
1
;
Lin-Lin CAO
1
;
Chang GUO
1
;
Yi-Heng WANG
2
;
Dan LIU
3
;
Jia-Hui SUN
2
;
Sheng WANG
2
;
Gang-Min ZHANG
1
;
Wen-Pan DONG
1
Author Information
1. School of Ecology and Nature Conservation, Beijing Forestry University Beijing 100083, China.
2. State Key Laboratory for Quality Ensurance and Sustainable Use of Dao-di Herbs, National Resource Center for Chinese Materia Medica, China Academy of Chinese Medical Sciences Beijing 100700, China.
3. Shandong Forest and Grass Germplasm Resource Center Ji'nan 250102, China.
- Publication Type:Journal Article
- Keywords:
Forsythia suspensa;
chloroplast genome;
genetic variation;
population structure
- MeSH:
Genome, Chloroplast;
Forsythia/classification*;
Phylogeny;
Genetic Variation;
Chloroplasts/genetics*;
Microsatellite Repeats
- From:
China Journal of Chinese Materia Medica
2025;50(8):2108-2115
- CountryChina
- Language:Chinese
-
Abstract:
Forsythia suspensa is a traditional Chinese medicine and a commonly used landscaping plant. Its dried fruit is used in medicine for its functions of clearing heat, removing toxins, reducing swelling, dissipating masses, and dispersing wind and heat. It possesses extremely high medicinal and economic value. However, the genetic differentiation and diversity of its wild populations remain unclear. In this study, chloroplast genome sequences were obtained from 15 wild individuals of F. suspensa using high-throughput sequencing technology. The sequence characteristics and intraspecific variations were analyzed. The results were as follows:(1) The full length of the F. suspensa chloroplast genome ranged from 156 184 to 156 479 bp, comprising a large single-copy region, a small single-copy region, and two inverted repeat regions. The chloroplast genome encoded a total of 132 genes, including 87 protein-coding genes, 37 tRNA genes, and 8 rRNA genes.(2) A total of 166-174 SSR loci, 792 SNV loci, and 63 InDel loci were identified in the F. suspensa chloroplast genome, indicating considerable genetic variation among individuals.(3) Population structure analysis revealed that F. suspensa could be divided into five or six groups. Both the population structure analysis and phylogenetic reconstruction results indicated significant genetic variation within the wild populations of F. suspensa, with no obvious correlation between intraspecific genetic differentiation and geographical distribution. This study provides new insights into the genetic diversity and differentiation within F. suspensa species and offers additional references for the conservation of species diversity and the utilization of germplasm resources in wild F. suspensa.
