Exploring in vivo existence forms of Notoginseng Radix et Rhizoma in rats.
10.19540/j.cnki.cjcmm.20250201.201
- Author:
Meng-Ge FENG
1
;
Lin-Han XIANG
2
;
Jing ZHANG
2
;
Wen-Hui ZHAO
1
;
Yang LI
2
;
Li-Li LI
3
;
Guang-Xue LIU
2
;
Shao-Qing CAI
1
;
Feng XU
1
Author Information
1. Medical College, Tibet University Lasa 850002, China Division of Pharmacognosy, School of Pharmaceutical Sciences, Peking University Beijing 100191, China.
2. Division of Pharmacognosy, School of Pharmaceutical Sciences, Peking University Beijing 100191, China.
3. Hebei Yuzhilin Pharmaceutical Co., Ltd. Shijiazhuang 050035, China.
- Publication Type:Journal Article
- Keywords:
Notoginseng Radix et Rhizoma;
UHPLC-Q-TOF-MS/MS;
effective forms;
in vivo metabolism;
saponin
- MeSH:
Animals;
Rats;
Drugs, Chinese Herbal/pharmacokinetics*;
Rhizome/metabolism*;
Male;
Rats, Sprague-Dawley;
Chromatography, High Pressure Liquid;
Panax notoginseng/chemistry*;
Tandem Mass Spectrometry;
Feces/chemistry*
- From:
China Journal of Chinese Materia Medica
2025;50(9):2539-2562
- CountryChina
- Language:Chinese
-
Abstract:
The study aims to elucidate the existence forms(original constituents and metabolites) of Notoginseng Radix et Rhizoma in rats and reveal its metabolic pathways. After Notoginseng Radix et Rhizoma was administered orally once a day for seven consecutive days to rats, all urine and feces samples were collected for seven days, while the blood samples were obtained 6 h after the last administration. Using the ultra high performance liquid chromatography-quadrupole time-of-flight tandem mass spectrometry(UHPLC-Q-TOF-MS/MS) technique, this study identified 6, 73, and 156 existence forms of Notoginseng Radix et Rhizoma in the rat plasma, urine, and feces samples, respectively. Among them, 101 compounds were identified as new existence forms, and 13 original constituents were identified by comparing with reference compounds. The metabolic reactions of constituents from Notoginseng Radix et Rhizoma were mainly deglycosylation, dehydration, hydroxylation, hydrogenation, dehydrogenation, acetylation, and amino acid conjugation. Furthermore, the possible in vivo metabolic pathways of protopanaxatriol(PPT) in rats were proposed. Through comprehensive analysis of the liquid chromatography-mass spectrometry(LC-MS) data, isomeric compounds were discriminated, and the planar chemical structures of 32 metabolites were clearly identified. According to the literature, 48 original constituents possess antitumor and cardiovascular protective bioactivities. Additionally, 32 metabolites were predicted to have similar bioactivities by SuperPred. This research lays the foundation for further exploring the in vivo effective forms of Notoginseng Radix et Rhizoma.
