Chemical constituents of bulbs of Narcissus tazetta var. chinensis.
10.19540/j.cnki.cjcmm.20250131.202
- Author:
Ling-Xia XU
1
;
Xin-Xin HUANG
1
;
Ji-Cheng SHU
2
;
Ting TAN
1
;
Yun LUO
2
Author Information
1. the National Pharmaceutical Engineering Center for Solid Preparation in Chinese Herbal Medicine, Jiangxi University of Chinese Medicine Nanchang 330006, China.
2. Key Laboratory of Modern Preparation of Traditional Chinese Medicine, Ministry of Education, Jiangxi University of Chinese Medicine Nanchang 330004, China.
- Publication Type:Journal Article
- Keywords:
anti-inflammatory activity;
bulbs of Narcissus tazetta var.chinensis;
isolation and identification;
narcissus A;
narcissus B
- MeSH:
Mice;
Animals;
Narcissus/chemistry*;
Drugs, Chinese Herbal/isolation & purification*;
Plant Roots/chemistry*;
Chromatography, High Pressure Liquid;
Macrophages/immunology*;
RAW 264.7 Cells
- From:
China Journal of Chinese Materia Medica
2025;50(9):2404-2410
- CountryChina
- Language:Chinese
-
Abstract:
The 95% ethanol extract from bulbs of Narcissus tazetta var. chinensis(BNTC) was eluted with 30%, 60%, and pure methanol on D-101 macroporous resin. The elution fractions were isolated and purified by silica gel column chromatography, thin layer chromatography, D-101 macroporous resin, semi-preparative high performance liquid chromatography(HPLC), and HPLC. The purified compounds were identified using one-dimensional and two-dimensional spectroscopy, high-resolution mass spectrometry, and other techniques. A total of 15 compounds were isolated and identified as 5-(4-hydroxy-3-methoxyphenyl)-3-(4-hydroxyphenyl)-N-methyl-3,6-dihydropyridine-2(1H)-one(1), 3,5-di(hydroxyphenyl)-N-methyl-3,6-dihydropyridine-2(1H)-one(2), protocatechualdehyde(3), protocatechuic acid(4), 3,4-dihydroxyacetophenone(5), syringic acid(6), vanillic acid(7), p-hydroxybenzoic acid(8),(2S)-4'-hydroxy-7-methoxyflavan(9), 2,4,6-trimethoxyacetophenone(10), N-trans-ferulic acid p-hydroxyphenylethylamine(11), N-cis-p-coumaroyltyramine(12), N-trans-p-coumaroyltyramine(13), piscidic acid(14), 5-hydroxymethylfurfural(15). Compounds 1 and 2 are new compounds with similar structure that have not been reported yet, named narcissus A and narcissus B. Compounds 8-13 were isolated and identified from the genus Narcissus for the first time, and compounds 14 and 15 were isolated from BNTC for the first time. Compounds 1 and 2 inhibited the release of NO from RAW264.7 cells induced by lipopolysaccharide(LPS)(P<0.001), with compound 1 having an IC_(50) value of(72.76±2.97) μmol·L~(-1) and compound 2 having an IC_(50) value of(63.59±0.96) μmol·L~(-1).
