Dehydrodiisoeugenol resists H1N1 virus infection via TFEB/autophagy-lysosome pathway.
10.19540/j.cnki.cjcmm.20241107.701
- Author:
Zhe LIU
1
;
Jun-Liang LI
2
;
Yi-Xiang ZHOU
2
;
Xia LIU
2
;
Yan-Li YU
1
;
Zheng LUO
1
;
Yao WANG
3
;
Xin JIA
1
Author Information
1. School of Chinese Materia Medica, Beijing University of Chinese Medicine Beijing 100029, China.
2. School of Life Science, Beijing University of Chinese Medicine Beijing 100029, China.
3. School of Life Science, Beijing University of Chinese Medicine Beijing 100029, China Beijing Institute of Traditional Chinese Medicine, Beijing University of Chinese Medicine Beijing 100029, China.
- Publication Type:Journal Article
- Keywords:
TFEB;
autophagy;
dehydrodiisoeugenol;
influenza A virus(H1N1)
- MeSH:
Influenza A Virus, H1N1 Subtype/genetics*;
Animals;
Autophagy/drug effects*;
Humans;
Mice;
Basic Helix-Loop-Helix Leucine Zipper Transcription Factors/genetics*;
Influenza, Human/metabolism*;
Lysosomes/metabolism*;
Orthomyxoviridae Infections/genetics*;
Eugenol/pharmacology*;
Antiviral Agents/pharmacology*;
Virus Replication/drug effects*;
A549 Cells;
Male
- From:
China Journal of Chinese Materia Medica
2025;50(6):1650-1658
- CountryChina
- Language:Chinese
-
Abstract:
The present study delves into the cellular mechanisms underlying the antiviral effects of dehydrodiisoeugenol(DEH) by focusing on the transcription factor EB(TFEB)/autophagy-lysosome pathway. The cell counting kit-8(CCK-8) was utilized to assess the impact of DEH on the viability of human non-small cell lung cancer cells(A549). The inhibitory effect of DEH on the replication of influenza A virus(H1N1) was determined by real-time quantitative polymerase chain reaction(RT-qPCR). Western blot was employed to evaluate the influence of DEH on the expression level of the H1N1 virus nucleoprotein(NP). The effect of DEH on the fluorescence intensity of NP was examined by the immunofluorescence assay. A mouse model of H1N1 virus infection was established via nasal inhalation to evaluate the therapeutic efficacy of 30 mg·kg~(-1) DEH on H1N1 virus infection. RNA sequencing(RNA-seq) was performed for the transcriptional profiling of mouse embryonic fibroblasts(MEFs) in response to DEH. The fluorescent protein-tagged microtubule-associated protein 1 light chain 3(LC3) was used to assess the autophagy induced by DEH. Western blot was employed to determine the effect of DEH on the autophagy flux of LC3Ⅱ/LC3Ⅰ under viral infection conditions. Lastly, the role of TFEB expression in the inhibition of DEH against H1N1 infection was evaluated in immortalized bone marrow-derived macrophage(iBMDM), both wild-type and TFEB knockout. The results revealed that the half-maximal inhibitory concentration(IC_(50)) of DEH for A549 cells was(87.17±0.247)μmol·L~(-1), and DEH inhibited H1N1 virus replication in a dose-dependent manner in vitro. Compared with the H1N1 virus-infected mouse model, the treatment with DEH significantly improved the body weights and survival time of mice. DEH induced LC3 aggregation, and the absence of TFEB expression in iBMDM markedly limited the ability of DEH to counteract H1N1 virus replication. In conclusion, DEH exerts its inhibitory activity against H1N1 infection by activating the TFEB/autophagy-lysosome pathway.
