Phenylpropanoids from roots of Berberis polyantha.
10.19540/j.cnki.cjcmm.20241201.201
- Author:
Dong-Mei SHA
1
;
Shuai-Cong NI
1
;
Li-Niu SHA-MA
1
;
Hai-Xiao-Lin-Mo MA
1
;
Xiao-Yong HE
1
;
Bin HE
1
;
Shao-Shan ZHANG
2
;
Ying LI
1
;
Jing WEN
3
;
Yuan LIU
2
;
Xin-Jia YAN
2
Author Information
1. Tibetan Plateau Ethnic Medicinal Resources Protection and Utilization Key Laboratory of National Ethnic Affairs Commission of the People's Republic of China Chengdu 610225, China Sichuan Provincial Qiang-Yi Medicinal Resources Protection and Utilization Technology Engineering Laboratory Chengdu 610225, China.
2. Tibetan Plateau Ethnic Medicinal Resources Protection and Utilization Key Laboratory of National Ethnic Affairs Commission of the People's Republic of China Chengdu 610225, China Sichuan Provincial Qiang-Yi Medicinal Resources Protection and Utilization Technology Engineering Laboratory Chengdu 610225, China College of Grassland Resources, Southwest Minzu University Chengdu 610041, China.
3. School of Pharmacy, Sichuan College of Traditional Chinese Medicine Mianyang 621000, China.
- Publication Type:Journal Article
- Keywords:
Berberis;
Berberis polyantha;
anti-inflammatory activity;
chemical constituent;
phenylpropanoid
- MeSH:
Plant Roots/chemistry*;
Animals;
Mice;
Berberis/chemistry*;
RAW 264.7 Cells;
Macrophages/immunology*;
Drugs, Chinese Herbal/isolation & purification*;
Nitric Oxide/metabolism*;
Molecular Structure;
Anti-Inflammatory Agents/isolation & purification*
- From:
China Journal of Chinese Materia Medica
2025;50(6):1564-1568
- CountryChina
- Language:Chinese
-
Abstract:
The chemical constituents were systematically separated from the roots of Berberis polyantha by various chromatographic methods, including silica gel column chromatography, HP20 column chromatography, polyamide column chromatography, reversed-phase C_(18) column chromatography, and preparative high-performance liquid chromatography. The structures of the compounds were identified by physicochemical properties and spectroscopic techniques(1D NMR, 2D NMR, UV, MS, and CD). Four phenylpropanoids were isolated from the methanol extract of the roots of B. polyantha, and they were identified as(2R)-1-(4-hydroxy-3,5-dimethoxyphenyl)-1-propanone-O-β-D-glucopyranoside(1), methyl 4-hydroxy-3,5-dimethoxybenzoate(2),(+)-syringaresinol(3), and syringaresinol-4-O-β-D-glucopyranoside(4). Compound 1 was a new compound, and other compounds were isolated from this plant for the first time. The anti-inflammatory activity of these compounds was evaluated based on the release of nitric oxide(NO) in the culture of lipopolysaccharide(LPS)-induced RAW264.7 macrophages. At a concentration of 10 μmol·L~(-1), all the four compounds inhibited the LPS-induced release of NO in RAW264.7 cells, demonstrating potential anti-inflammatory properties.
