Two new protoberberine alkaloids from Stephania hernandifolia.
10.19540/j.cnki.cjcmm.20241223.201
- Author:
Wei-Hua DAI
1
;
Xin-Tao CUI
1
;
Yu-Jiao TU
1
;
Lei JIANG
1
;
Lin YUAN
1
Author Information
1. School of Chemistry and Chemical Engineering, Kunming University Kunming 650214, China.
- Publication Type:Journal Article
- Keywords:
Stephania hernandifolia;
amide alkaloids;
inhibitory activity against α-glucosidase;
protoberberine alkaloid
- MeSH:
Berberine Alkaloids/isolation & purification*;
Stephania/chemistry*;
Drugs, Chinese Herbal/isolation & purification*;
Molecular Structure;
alpha-Glucosidases/metabolism*;
Chromatography, High Pressure Liquid;
Alkaloids/isolation & purification*
- From:
China Journal of Chinese Materia Medica
2025;50(5):1231-1235
- CountryChina
- Language:Chinese
-
Abstract:
The 95% ethanol extract of Stephania hernandifolia was isolated and purified by column chromatography on silica gel and Sephadex LH-20, RP-18 medium-pressure liquid chromatography, and semi-preparative high performance liquid chromatography. The chemical structures of the compounds were identified by NMR and high-resolution mass spectrometry. Four alkaloids were isolated and identified as(-)-8-oxo-2,3,4,10,11-pentamethoxyberberine(1),(-)-8-oxo-11-hydroxy-2,3,4,10-tetramethoxyberberine(2), N-trans-feruloyl tyramine(3), and N-cis-feruloyl tyramine(4). Compounds 1 and 2 were new protoberberine alkaloids, while compounds 3 and 4 were amide alkaloids. All the four compounds were separated from this plant for the first time. The inhibitory activities of compounds 1, 3, and 4 against α-glycosidase were measured by the enzymatic reaction in vitro with 4-nitrophenyl-α-D-glucopyranoside(PNPG) as the substrate. Compounds 3 and 4 showed inhibitory activities against α-glucosidase, with median inhibition concentration(IC_(50)) values of(7.09±0.42) and(31.25±1.14) μmol·L~(-1), respectively.
