Pharmacokinetics and tissue distribution of fluorescent-labeled Astragalus polysaccharides in mice.
10.19540/j.cnki.cjcmm.20250111.201
- Author:
Xiao-Huan WANG
1
;
Peng-Xin LI
2
;
Ting-Ting GONG
2
;
Yun-Qian LU
2
;
Bo YANG
3
;
Xiang-Tao WANG
1
Author Information
1. College of Pharmacy, Harbin University of Commerce Harbin 150076, China Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences & Peking Union Medical College Beijing 100193, China.
2. Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences & Peking Union Medical College Beijing 100193, China.
3. College of Pharmacy, Harbin University of Commerce Harbin 150076, China.
- Publication Type:Journal Article
- Keywords:
Astragalus polysaccharides;
IR783;
pharmacokinetics;
tissue distribution
- MeSH:
Animals;
Mice;
Polysaccharides/chemistry*;
Tissue Distribution;
Astragalus Plant/chemistry*;
Male;
Drugs, Chinese Herbal/chemistry*;
Fluorescent Dyes/pharmacokinetics*;
Female
- From:
China Journal of Chinese Materia Medica
2025;50(7):1959-1968
- CountryChina
- Language:Chinese
-
Abstract:
In this study, the reductive amination method was used to label IR783 on Astragalus polysaccharides(APS) for the first time, which was verified by ultraviolet-visible spectroscopy and infrared spectroscopy. Quantitative analysis methods of APS-IR783 in plasma and various tissue were established using a multifunctional microplate reader. The pharmacokinetics and tissue distribution of APS-IR783 in mice were investigated after a single intravenous injection of 30 mg·kg~(-1) APS-IR783, and pharmacokinetic parameters were calculated using DAS 2.0 software. The results showed that the APS used had a mass fraction of 93.69%, a relative molecular weight of 1.55×10~5, and a polydispersity index(PDI, M_w/M_n) of 1.73, close to a homogeneous polysaccharide. The IR783 labeling yield reached 86.50%, and the content of IR783 in APS-IR783 was 0.72%. After a single intravenous injection of 30 mg·kg~(-1), the pharmacokinetic parameters of APS in mouse plasma were as follows: T_(max) was(0.67±0.26) h; C_(max) was(1 599.29±159.30) mg·L~(-1); T_(1/2α) and T_(1/2β) were(2.29±3.06) h and(0.44±0.05) h, respectively; AUC_(0-t) was(23 398.91±2 907.03) mg·h·L~(-1); AUC_(0-∞) was(27 710.55±3 506.55) mg·h·L~(-1); MRT_(0-∞) was(34.38±12.59) h; CL was 0.001 L·h~(-1)·kg~(-1); V_z was(0.042±0.017) L·kg~(-1). The in vivo biodistribution study demonstrated that the in vivo exposure ratios of APS in different tissue were in the following order: spleen > liver > kidney > lung > heart > small intestine > muscle > large intestine > brain > stomach, where the top five tissue accounted for 87.54% of the total area under the curve(AUC). This study successfully labeled APS with a water-soluble near-infrared fluorescent probe of IR783 for the first time and revealed the pharmacokinetics and tissue distribution of APS in mice. The paper provides detailed in vivo behavior of APS after intravenous injection, which lays the foundation for the development and utilization of APS and related natural medicines.
