Effect of Biyan Jiedu Capsules on proliferation and apoptosis of nasopharyngeal carcinoma cells based on PI3K/Akt pathway.
10.19540/j.cnki.cjcmm.20250109.502
- Author:
Ting LIN
1
;
Yang-Yang TAO
2
;
Ying-Gang TANG
3
;
Ju YUAN
4
;
Hui-Ping DU
5
;
Lin-Yu DENG
6
;
Fang-Liang ZHOU
7
;
Ying-Chun HE
7
Author Information
1. Hunan Provincial Key Laboratory for the Prevention and Treatment of Ophthalmology and Otolaryngology Diseases with Traditional Chinese Medicine, Hunan University of Chinese Medicine Changsha 410208, China Hunan Provincial Engineering and Technological Research Center for Prevention and Treatment of Ophthalmology and Otolaryngology Diseases with Chinese Medicine and Protecting Visual Function, Hunan University of Chinese Medicine Changsha 410208, China School of Traditional Chinese Medicine, Hunan University of Chinese Medicine Changsha 410208, China.
2. Medical School, Hunan University of Chinese Medicine Changsha 410208, China.
3. the First Hospital of Hunan University of Chinese Medicine Changsha 410007, China.
4. School of Traditional Chinese Medicine, Hunan University of Chinese Medicine Changsha 410208, China.
5. School of Integrated Chinese and Western Medicine, Hunan University of Chinese Medicine Changsha 410208, China.
6. School of Acupuncture and Tuina, Hunan University of Chinese Medicine Changsha 410208, China.
7. Hunan Provincial Key Laboratory for the Prevention and Treatment of Ophthalmology and Otolaryngology Diseases with Traditional Chinese Medicine, Hunan University of Chinese Medicine Changsha 410208, China Hunan Provincial Engineering and Technological Research Center for Prevention and Treatment of Ophthalmology and Otolaryngology Diseases with Chinese Medicine and Protecting Visual Function, Hunan University of Chinese Medicine Changsha 410208, China Medical School, Hunan University of Chinese Medicine Changsha 410208, China.
- Publication Type:Journal Article
- Keywords:
Biyan Jiedu Capsules;
PI3K/Akt pathway;
cell apoptosis;
cell proliferation;
nasopharyngeal carcinoma
- MeSH:
Humans;
Apoptosis/drug effects*;
Cell Proliferation/drug effects*;
Nasopharyngeal Carcinoma;
Nasopharyngeal Neoplasms/physiopathology*;
Proto-Oncogene Proteins c-akt/genetics*;
Cell Line, Tumor;
Drugs, Chinese Herbal/pharmacology*;
Phosphatidylinositol 3-Kinases/genetics*;
Signal Transduction/drug effects*;
Capsules;
Carcinoma/drug therapy*
- From:
China Journal of Chinese Materia Medica
2025;50(7):1920-1927
- CountryChina
- Language:Chinese
-
Abstract:
To investigate the effects of Biyan Jiedu Capsules on the proliferation and apoptosis of nasopharyngeal carcinoma cells and their molecular mechanism, nasopharyngeal carcinoma cells CNE1 and CNE2 were used. They were divided into control group(30% blank serum medium), low-(10% drug-containing serum + 20% blank serum medium), medium-(20% drug-containing serum + 10% blank serum medium), and high-(30% drug-containing serum medium) concentration group of Biyan Jiedu Capsules according to in vitro experiment. After 24 h of intervention, the effects of Biyan Jiedu Capsules on the proliferation of CNE1 and CNE2 were detected by CCK-8 assay, clonal formation experiment, and EdU staining. The effect of Biyan Jiedu Capsules on apoptosis of CNE1 and CNE2 was detected by flow cytometry. Western blot was used to detect the effect of Biyan Jiedu Capsules on the expression of X-linked apoptosis inhibitor protein(XIAP), survivin, proliferating cell nuclear antigen(PCNA), and PI3K/Akt pathway-related proteins in CNE1 and CNE2. The results showed that compared with the control group, the survival rate of CNE1 and CNE2 in the medium and high concentration groups of Biyan Jiedu Capsules could be decreased in a concentration-dependent way(P<0.05, P<0.01). At the same time, EdU staining and clonal formation experiments showed that the proliferation of CNE1 and CNE2 was significantly inhibited in the medium and high concentration groups of Biyan Jiedu Capsules(P<0.05, P<0.01). Flow cytometry showed that the apoptosis rate of CNE1 and CNE2 was significantly increased in all concentration groups of Biyan Jiedu Capsules(P<0.01), and the apoptosis rate was concentration-dependent. Western blot showed that the expressions of XIAP, survivin, PCNA, p-PI3K, and p-Akt in all concentration groups of Biyan Jiedu Capsules were significantly down-regulated(P<0.05, P<0.01). In conclusion, Biyan Jiedu Capsules can inhibit the proliferation and induce apoptosis of nasopharyngeal carcinoma cells possibly by down-regulating the PI3K/Akt signaling pathway.
