Congrong San ameliorates cognitive impairment and neuroinflammation in rat model of Alzheimer's disease by alleviating endoplasmic reticulum stress to inhibit NLRP3 inflammasome activation.
10.19540/j.cnki.cjcmm.20241107.704
- Author:
Yuan-Qin CAI
1
;
Yang XIANG
2
;
Qing-Hua LONG
3
;
Xi WANG
1
;
Chu-Hua ZENG
2
Author Information
1. Medical School, Hubei Minzu University Enshi 445000, China Hubei Provincial Key Laboratory of Occurrence and Intervention of Rheumatic Diseases, Hubei Minzu University Enshi 445000, China.
2. School of Basic Medical Sciences, Yunnan University of Chinese Medicine Kunming 650500, China Key Laboratory of Traditional Chinese Medicine for the Prevention and Treatment of Neuropsychiatric Diseases of Yunnan Provincial Department of Education, Yunnan University of Chinese Medicine Kunming 650500, China Yunnan Provincial Key Laboratory of Integrated Traditional Chinese and Western Medicine for the Prevention and Treatment of Chronic Diseases, Yunnan University of Chinese Medicine Kunming 650500, China.
3. Medical School, Hubei Minzu University Enshi 445000, China.
- Publication Type:Journal Article
- Keywords:
Congrong San;
NLRP3 inflammasome;
endoplasmic reticulum stress;
neuroinflammation;
neuron damage
- MeSH:
Animals;
Endoplasmic Reticulum Stress/drug effects*;
Male;
Alzheimer Disease/psychology*;
NLR Family, Pyrin Domain-Containing 3 Protein/metabolism*;
Rats, Sprague-Dawley;
Rats;
Inflammasomes/genetics*;
Drugs, Chinese Herbal/administration & dosage*;
Cognitive Dysfunction/metabolism*;
Disease Models, Animal;
Hippocampus/drug effects*;
Humans;
Neuroinflammatory Diseases/drug therapy*
- From:
China Journal of Chinese Materia Medica
2025;50(7):1881-1888
- CountryChina
- Language:Chinese
-
Abstract:
This study aims to investigate the effect of Congrong San(CRS) on endoplasmic reticulum stress-induced neuroinflammation in the rat model of Aβ_(1-42)-induced Alzheimer's disease(AD). Sixty male Sprague-Dawley rats(2 months old) were randomized into blank(CON), model(MOD), low-dose Congrong San(L-CRS), medium-dose Congrong San(M-CRS), high-dose Congrong San(H-CRS), and memantine hydrochloride(MJG) groups. The Morris water maze test was carried out to examine the learning and memory abilities of rats in each group. Hematoxylin-eosin staining and Nissl staining were employed to observe the morphology and number of CA1 neurons in the hippocampus of rats in each group. The morphology and structure of the endoplasmic reticulum in the hippocampus were observed by transmission electron microscopy. The immunofluorescence assay was employed to detect the expression of 78 kDa glucose-regulated protein(GRP78) in the hippocampus. Western blot was employed to determine the expression of apoptosis-associated speck-like protein containing a CARD(ASC), cysteinyl aspartate-specific proteinase(caspase-1), interleukin-18(IL-18), interleukin-1β(IL-1β), GRP78, and pathway proteins including protein kinase RNA-like endoplasmic reticulum kinase(PERK), phosphorylated PERK(p-PERK), C/EBP homologous protein(CHOP), and NOD-like receptor pyrin domain-containing protein 3(NLRP3) in the rat hippocampus. Compared with the MOD group, the M-CRS and H-CRS groups showed improved learning and memory abilities, reduced neuron losses in the hippocampus, alleviated endoplasmic reticulum stress, inhibited PERK-CHOP-NLRP3 pathway, and lowered levels of IL-1β, IL-6, and tumor necrosis factor-alpha(TNF-α). The results suggest that CRS can alleviate cognitive impairment and hippocampal neuron damage and reduce neuroinflammation in AD rats by alleviating endoplasmic reticulum stress to inhibit the activation of NLRP3 inflammasomes.
