Mechanism of Colquhounia Root Tablets against diabetic kidney disease via RAGE-ROS-PI3K-AKT-NF-κB-NLRP3 signaling axis.
10.19540/j.cnki.cjcmm.20250110.401
- Author:
Ming-Zhu XU
1
;
Zhao-Chen MA
2
;
Zi-Qing XIAO
1
;
Shuang-Rong GAO
2
;
Yi-Xin YANG
3
;
Jia-Yun SHEN
2
;
Chu ZHANG
3
;
Feng HUANG
4
;
Jiang-Rui WANG
5
;
Bei-Lei CAI
6
;
Na LIN
2
;
Yan-Qiong ZHANG
1
Author Information
1. Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences Beijing 100700, China College of Traditional Chinese Medicine, Yunnan University of Chinese Medicine Kunming 650500, China.
2. Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences Beijing 100700, China.
3. Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences Beijing 100700, China College of Pharmacy, Fujian University of Traditional Chinses Medicine Fuzhou 350122, China.
4. College of Traditional Chinese Medicine, Yunnan University of Chinese Medicine Kunming 650500, China.
5. Pharmaceutical Factory of the Chongqing Academy of Chinese Materia Medica Chongqing 400800, China Sinomune Pharmaceutical Co., Ltd. Wuxi 214194, China.
6. Sinomune Pharmaceutical Co., Ltd. Wuxi 214194, China.
- Publication Type:Journal Article
- Keywords:
Colquhounia Root Tablets;
RAGE-ROS-PI3K-AKT-NF-κB-NLRP3 signaling axis;
diabetic kidney disease;
immune-inflammation;
metabolic reprogramming
- MeSH:
Animals;
Diabetic Nephropathies/metabolism*;
Receptor for Advanced Glycation End Products/genetics*;
NF-kappa B/genetics*;
Signal Transduction/drug effects*;
Rats;
NLR Family, Pyrin Domain-Containing 3 Protein/genetics*;
Proto-Oncogene Proteins c-akt/genetics*;
Drugs, Chinese Herbal/administration & dosage*;
Male;
Phosphatidylinositol 3-Kinases/genetics*;
Reactive Oxygen Species/metabolism*;
Humans;
Plant Roots/chemistry*;
Rats, Sprague-Dawley;
Tablets/administration & dosage*
- From:
China Journal of Chinese Materia Medica
2025;50(7):1830-1840
- CountryChina
- Language:Chinese
-
Abstract:
This study aimed to explore the therapeutic mechanisms of Colquhounia Root Tablets(CRT) in treating diabetic kidney disease(DKD) by integrating biomolecular network mining with animal model verification. By analyzing clinical transcriptomics data, an interaction network was constructed between candidate targets of CRT and DKD-related genes. Based on the topological eigenvalues of network nodes, 101 core network targets of CRT against DKD were identified. These targets were found to be closely related to multiple pathways associated with type 2 diabetes, immune response, and metabolic reprogramming. Given that immune-inflammatory imbalance driven by metabolic reprogramming is one of the key pathogenic mechanisms of DKD, and that many core network targets of CRT are involved in this pathological process, receptor for advanced glycation end products(RAGE)-reactive oxygen species(ROS)-phosphatidylinositol 3-kinase(PI3K)-protein kinase B(AKT)-nuclear factor-κB(NF-κB)-NOD-like receptor family pyrin domain containing 3(NLRP3) signaling axis was selected as a candidate target for in-depth research. Further, a rat model of DKD induced by a high-sugar, high-fat diet and streptozotocin was established to evaluate the pharmacological effects of CRT and verify the expression of related targets. The experimental results showed that CRT could effectively correct metabolic disturbances in DKD, restore immune-inflammatory balance, and improve renal function and its pathological changes by inhibiting the activation of the RAGE-ROS-PI3K-AKT-NF-κB-NLRP3 signaling axis. In conclusion, this study reveals that CRT alleviates the progression of DKD through dual regulation of metabolic reprogramming and immune-inflammatory responses, providing strong experimental evidence for its clinical application in DKD.
