Antipyretic effects of ethanol extracts of Arisaematis Rhizoma fermented with bile from different sources.
10.19540/j.cnki.cjcmm.20250113.303
- Author:
Run ZOU
1
;
Fa-Zhi SU
1
;
En-Lin ZHU
2
;
Chen-Xi BAI
1
;
Yan-Ping SUN
1
;
Hai-Xue KUANG
1
;
Qiu-Hong WANG
3
Author Information
1. Key Laboratory of Basic and Application Research of Beiyao, Ministry of Education, Heilongjiang University of Chinese Medicine Harbin 150040, China.
2. Clinical Medical College of Acupuncture Moxibustion and Rehabilitation, Guangzhou University of Chinese Medicine Guangzhou 510006, China.
3. Key Laboratory of Basic and Application Research of Beiyao, Ministry of Education, Heilongjiang University of Chinese Medicine Harbin 150040, China Department of Chinese Materia Medica Processing, School of Traditional Chinese Medicine,Guangdong Pharmaceutical University Guangzhou 510006, China.
- Publication Type:Journal Article
- Keywords:
Arisaema Cum Bile;
antipyretic effect;
bile from different sources;
inflammation;
metabolomics
- MeSH:
Animals;
Rats;
Male;
Fermentation;
Rats, Sprague-Dawley;
Rhizome/metabolism*;
Drugs, Chinese Herbal/chemistry*;
Bile/chemistry*;
Antipyretics/chemistry*;
Fever/metabolism*;
Cattle;
Swine;
Tumor Necrosis Factor-alpha/metabolism*;
Ethanol/chemistry*;
Interleukin-6/blood*;
Interleukin-1beta/blood*
- From:
China Journal of Chinese Materia Medica
2025;50(7):1781-1791
- CountryChina
- Language:Chinese
-
Abstract:
This study aims to investigate the antipyretic effects and mechanisms of ethanol extracts from Arisaematis Rhizoma fermented with bile from different sources on a rat model of fever induced by a dry-yeast suspension. The rat model of fever was established by subcutaneous injection of 20% dry-yeast suspension into the rat back. The levels of tumor necrosis factor-α(TNF-α), interleukin-1β(IL-1β), interleukin-6(IL-6) in the serum, as well as prostaglandin E_2(PGE_2) and cyclic adenosine monophosphate(cAMP) in the hypothalamus, were determined by ELISA. Metabolomics analysis was then performed on serum and hypothalamus samples based on UPLC-Q-TOF MS to explore the potential biomarkers and metabolic pathways. The results showed that the body temperatures of rats significantly rose 4 h after modeling. After oral administration of high-dose ethanol extracts of Arisaematis Rhizoma fermented with bovine bile(NCH) and porcine bile(ZCH), the body temperatures of rats declined(P<0.05), and the NCH group showed better antipyretic effect than the ZCH group. Additionally, compared with the model group, the NCH and ZCH groups showed lowered levels of IL-1β, IL-6, TNF-α, PGE_2, and cAMP(P<0.01). The results of serum and hypothalamus metabolomics analysis indicated that both NCH and ZCH exerted antipyretic effects by regulating phenylalanine metabolism, sphingolipid metabolism, arachidonic acid metabolism, and steroid hormone biosynthesis. Collectively, both NCH and ZCH can play an obvious antipyretic role in the rat model of dry yeast-induced fever, and the underlying mechanism might be closely associated with inhibiting inflammation and regulating metabolic disorders. Moreover, NCH demonstrates better antipyretic effect.
