Detection and sequence analysis of broad bean wilt virus 2 on Rehmannia glutinosa.
10.19540/j.cnki.cjcmm.20250115.103
- Author:
Xiao-Long DENG
1
;
Jie YAO
2
;
Lang QIN
1
;
Shi-Wen DING
3
;
Tie-Lin WANG
4
;
Kun ZHANG
1
;
Lei CHENG
2
;
Zhen HE
1
Author Information
1. College of Plant Protection, Yangzhou University Yangzhou 225009, China.
2. Joint Research Center for Chinese Herbal Medicine of Anhui of IHM, Bozhou Vocational and Technical College Bozhou 236800, China Anhui Huatuo Chinese Medicine Research Institute Bozhou 236800, China.
3. Rural Revitalization Faculty, Hengyang Technician College Hengyang 421101, China.
4. State Key Laboratory for Quality Ensurance and Sustainable Use of Dao-di Herbs, National Resource Center for Chinese Materia Medica, China Academy of Chinese Medical Sciences Beijing 100700, China.
- Publication Type:Journal Article
- Keywords:
Rehmannia glutinosa;
broad bean wilt virus 2;
diversity;
phylogenetic analysis
- MeSH:
Rehmannia/virology*;
Phylogeny;
Plant Diseases/virology*;
China;
Molecular Sequence Data;
Fabavirus/classification*
- From:
China Journal of Chinese Materia Medica
2025;50(7):1741-1747
- CountryChina
- Language:Chinese
-
Abstract:
To clarify the occurrence and distribution of broad bean wilt virus 2(BBWV2) on Rehmannia glutinosa, this study collected 87 R. glutinosa samples with typical symptoms of viral disease such as chlorosis and crumple from Wenxian county and Wuzhi county in Jiaozuo city, Henan province and Qiaocheng district in Bozhou city, Anhui province. The BBWV2 CP target band was amplified from 37 R. glutinosa samples by RT-PCR technology. The total detection rate reached 42.5%, among which 43.0% was detected in samples from Henan province. The detection rate in samples from Anhui province was 37.5%. 37 BBWV2 CP sequences were obtained by cloning and sequencing of BBWV2 positive samples(data has been submitted to GenBank, accession numbers: PP407959-PP407995), and the sequence analysis of these CP sequences with 91 other BBWV2 isolates in GenBank showed a high genetic diversity with a consistency rate of 70.8%-100%. Meanwhile, phylogenetic analysis showed that BBWV2 could be divided into three groups according to CP sequences, among which the BBWV2 in R. glutinosa isolates obtained in this study were all located in group 3. This study identified the differences in the occurrence, distribution, and genetic diversity of BBWV2 in R. glutinosa from Henan province and Anhui province and provided a theoretical basis for the prevention and control of BBWV2.
