Lipid-lowering activity of Panax notoginseng flowers and rhizomes on hyperlipidemia rats based on chemical composition similarity.
10.19540/j.cnki.cjcmm.20241014.501
- Author:
Meng YE
1
;
Jin-Wen MA
1
;
Hai-Yue ZHONG
1
;
Yu-Ling XU
2
Author Information
1. School of Pharmacy, Chengdu University Chengdu 610106, China.
2. School of Pharmacy, Chengdu University Chengdu 610106, China Sichuan Antiviral Chinese Medicine Industrialization Engineering Technology Research Center Chengdu 610106, China.
- Publication Type:Journal Article
- Keywords:
Panax notoginseng;
Panax notoginseng flower;
hyperlipidemia;
intestinal flora;
lipid-lowering
- MeSH:
Animals;
Hyperlipidemias/microbiology*;
Panax notoginseng/chemistry*;
Rats;
Rhizome/chemistry*;
Male;
Flowers/chemistry*;
Drugs, Chinese Herbal/administration & dosage*;
Rats, Sprague-Dawley;
Hypolipidemic Agents/administration & dosage*;
Gastrointestinal Microbiome/drug effects*;
Humans;
Liver/drug effects*
- From:
China Journal of Chinese Materia Medica
2025;50(3):776-786
- CountryChina
- Language:Chinese
-
Abstract:
Based on the similarity of chemical constituents between Panax notoginseng flowers and rhizomes, this study investigated their lipid-lowering effects and impacts on the intestinal flora of rats. The main components of P. notoginseng flowers and rhizomes were detected by ultra-high-performance liquid chromatography-quadrupole time-of-flight mass spectrometry(UHPLC-Q-TOF-MS) to compare their chemical similarities. A hyperlipidemia rat model was induced using a high-fat diet. After successful modeling, the rats were divided into the blank control group, blank administration group(0.090 g·kg~(-1)), model group, low-(0.045 g·kg~(-1)), medium-(0.090 g·kg~(-1)), high-dose(0.180 g·kg~(-1)) P. notoginseng flower group, P. notoginseng rhizome group(0.270 g·kg~(-1)), and simvastatin group(0.900 mg·kg~(-1)). After modeling, the rats were given intragastric administration for 3 weeks, once daily, while their body weight was recorded regularly. Before the last administration, fresh feces were collected for analysis of changes in intestinal flora using 16S rDNA high-throughput sequencing technology. One hour after the last administration, the rats were anesthetized with 1% pentobarbital sodium, and blood was collected from the abdominal aorta. Serum biochemical indexes were detected using an automatic biochemical analyzer. Organs(heart, liver, spleen, lung, and kidney) were harvested, and organ index were calculated. Liver tissue pathology was assessed through HE staining and oil red O staining. The results indicated that there were 33 identical chemical constituents in P. notoginseng flowers and rhizomes, accounting for 75.00% of the total constituents. After treatment, high-dose P. notoginseng flower group and P. notoginseng rhizome group exhibited similar effects on body weight, serum biochemical indexes, and liver histopathological conditions. Compared with model control group, the abundance of Firmicutes and Actinobacteria increased in high-dose P. notoginseng flower and rhizome groups, while the abundance of Bacteroidetes and Thermodesulfobacteria decreased. Cluster analysis showed no significant difference between the two groups. Both P. notoginseng flowers and rhizomes possess similar chemical components and lipid-lowering effects, and they can regulate the intestinal flora imbalance caused by hyperlipidemia, indicating their potential for use in hyperlipidemia treatment.
