Effects of Rehmanniae Radix Praeparata on striatal neuronal apoptosis in ADHD rats via Bcl-2/Bax/caspase-3 pathway.
10.19540/j.cnki.cjcmm.20240802.706
- Author:
Jing WANG
1
;
Kang-Lin ZHU
1
;
Xin-Qiang NI
2
;
Wen-Hua CAI
1
;
Yu-Ting YANG
1
;
Jia-Qi ZHANG
3
;
Chong ZHOU
1
;
Mei-Jun SHI
4
Author Information
1. the Fourth Clinical Medical College of Guangzhou University of Chinese Medicine Shenzhen 518033, China.
2. the Fourth Clinical Medical College of Guangzhou University of Chinese Medicine Shenzhen 518033, China Shenzhen Traditional Chinese Medicine Hospital Shenzhen 518033, China.
3. the Seventh Affiliated Hospital of Sun Yat-sen University Shenzhen 518107, China.
4. Shenzhen Traditional Chinese Medicine Hospital Shenzhen 518033, China.
- Publication Type:Journal Article
- Keywords:
Bax;
Bcl-2;
Rehmanniae Radix Praeparata;
attention deficit hyperactivity disorder;
caspase-3;
neuronal apoptosis;
striatum
- MeSH:
Animals;
Male;
Apoptosis/drug effects*;
Rats;
Drugs, Chinese Herbal/administration & dosage*;
Caspase 3/genetics*;
Proto-Oncogene Proteins c-bcl-2/genetics*;
bcl-2-Associated X Protein/genetics*;
Rehmannia/chemistry*;
Attention Deficit Disorder with Hyperactivity/physiopathology*;
Signal Transduction/drug effects*;
Neurons/cytology*;
Rats, Inbred SHR;
Rats, Inbred WKY;
Humans;
Corpus Striatum/cytology*;
Plant Extracts
- From:
China Journal of Chinese Materia Medica
2025;50(3):750-757
- CountryChina
- Language:Chinese
-
Abstract:
This study investigated the effects of Rehmanniae Radix Praeparata on striatal neuronal apoptosis in rats with attention deficit hyperactivity disorder(ADHD) based on the B-cell lymphoma-2(Bcl-2)/Bcl-2-associated X protein(Bax)/caspase-3 signaling pathway. Twenty-four 3-week-old male spontaneously hypertensive rats(SHR) were randomly divided into a model group, a methylphenidate group(2 mg·kg~(-1)·d~(-1)), and a Rehmanniae Radix Praeparata group(2.4 mg·kg~(-1)·d~(-1)). Age-matched male Wistar Kyoto(WKY) rats were used as the normal control group, with 8 rats in each group. The rats were administered by gavage for 28 days. Body weight and food intake were recorded for each group. The open field test and elevated plus maze test were used to assess hyperactivity and impulsive behaviors. Nissl staining was used to detect changes in striatal neurons and Nissl bodies. Terminal deoxynucleotidyl transferase dUTP nick end labeling(TUNEL) fluorescence staining was used to detect striatal cell apoptosis. Western blot was employed to detect the expression levels of Bcl-2, Bax, and caspase-3 proteins in the striatum. The results showed that compared with the model group, Rehmanniae Radix Praeparata significantly reduced the total movement distance, average movement speed, and central area residence time in the open field test, and significantly reduced the ratio of open arm entries, open arm stay time, and head dipping in the elevated plus maze test. Furthermore, it increased the number of Nissl bodies in striatal neurons, significantly downregulated the apoptosis index, significantly increased Bcl-2 protein expression and the Bcl-2/Bax ratio, and reduced Bax and caspase-3 protein expression. In conclusion, Rehmanniae Radix Praeparata can reduce hyperactivity and impulsive behaviors in ADHD rats. Its mechanism may be related to the regulation of the Bcl-2/Bax/caspase-3 signaling pathway in the striatum, enhancing the anti-apoptotic capacity of striatal neurons.
