Synergistic neuroprotective effects of main components of salvianolic acids for injection based on key pathological modules of cerebral ischemia.
10.19540/j.cnki.cjcmm.20241031.302
- Author:
Si-Yu TAN
1
;
Ya-Xu WU
1
;
Zi-Shu YAN
2
;
Ai-Chun JU
3
;
De-Kun LI
3
;
Peng-Wei ZHUANG
4
;
Yan-Jun ZHANG
5
;
Hong GUO
2
Author Information
1. Haihe Laboratory of Modern Chinese Medicine, Tianjin University of Traditional Chinese Medicine Tianjin 301617, China.
2. State Key Laboratory of Component-based Chinese Medicine, Tianjin University of Traditional Chinese Medicine Tianjin 301617, China.
3. Tianjin Tasly Pride Pharmaceutical Co., Ltd. Tianjin 300402, China.
4. Haihe Laboratory of Modern Chinese Medicine, Tianjin University of Traditional Chinese Medicine Tianjin 301617, China First Teaching Hospital of Tianjin University of Traditional Chinese Medicine Tianjin 300193, China.
5. First Teaching Hospital of Tianjin University of Traditional Chinese Medicine Tianjin 300193, China National Clinical Research Center for Chinese Medicine Acupuncture and Moxibustion Tianjin 300193, China.
- Publication Type:Journal Article
- Keywords:
endothelial injury;
ischemic stroke;
neuroinflammation;
salvianolic acids for injection;
synergistic effects of drug components
- MeSH:
Brain Ischemia/metabolism*;
Neuroprotective Agents/pharmacology*;
Animals;
Drugs, Chinese Herbal/administration & dosage*;
Benzofurans/pharmacology*;
Mice;
Drug Synergism;
Caffeic Acids/pharmacology*;
Polyphenols/pharmacology*;
Humans;
Alkenes/pharmacology*;
Endothelial Cells/drug effects*;
Depsides
- From:
China Journal of Chinese Materia Medica
2025;50(3):693-701
- CountryChina
- Language:Chinese
-
Abstract:
This study aims to explore the synergistic effects of the main components in salvianolic acids for Injection(SAFI) on key pathological events in cerebral ischemia, elucidating the pharmacological characteristics of SAFI in neuroprotection. Two major pathological gene modules related to endothelial injury and neuroinflammation in cerebral ischemia were mined from single-cell data. According to the topological distance calculated in network medicine, potential synergistic component combinations of SAFI were screened out. The results showed that the combination of caffeic acid and salvianolic acid B scored the highest in addressing both endothelial injury and neuroinflammation, demonstrating potential synergistic effects. The cell experiments confirmed that the combination of these two components at a ratio of 1∶1 significantly protected brain microvascular endothelial cells(bEnd.3) from oxygen-glucose deprivation/reoxygenation(OGD/R)-induced reperfusion injury and effectively suppressed lipopolysaccharide(LPS)-induced neuroinflammatory responses in microglial cells(BV-2). This study provides a new method for uncovering synergistic effects among active components in traditional Chinese medicine(TCM) and offers novel insights into the multi-component, multi-target acting mechanisms of TCM.
