Role of miR-140-5p/BCL2L1 in apoptosis and autophagy of HFOB1.19 and effect of Bushen Jianpi Huoxue Decoction.
10.19540/j.cnki.cjcmm.20240719.403
- Author:
Tong-Ying CHEN
1
;
Sai FU
1
;
Xiao-Yun LI
1
;
Shu-Hua LIU
1
;
Yi-Fu YANG
1
;
Dong-Sheng YANG
1
;
Yun-Jie ZENG
1
;
Yang-Bo LI
1
;
Dan LUO
2
;
Hong-Xing HUANG
3
;
Lei WAN
3
Author Information
1. Guangzhou University of Chinese Medicine Guangzhou 510006, China Laboratory of Orthopaedics and Traumatology, Lingnan Medical Research Centre, Guangzhou University of Chinese Medicine Guangzhou 510006, China.
2. the Second Affiliated Hospital of Guangzhou University of Chinese Medicine Guangzhou 510006, China.
3. the Third Affiliated Hospital of Guangzhou University of Chinese Medicine Guangzhou 510378, China.
- Publication Type:Journal Article
- Keywords:
BCL2L1;
Bushen Jianpi Huoxue Decoction;
apoptosis;
autophagy;
miR-140-5p;
osteoporosis
- MeSH:
MicroRNAs/metabolism*;
Autophagy/drug effects*;
Apoptosis/drug effects*;
Humans;
Drugs, Chinese Herbal/administration & dosage*;
Animals;
Cell Line;
bcl-X Protein/metabolism*;
Osteoblasts/metabolism*;
Rats;
Osteoporosis/physiopathology*;
Male;
Rats, Sprague-Dawley;
Osteogenesis/drug effects*
- From:
China Journal of Chinese Materia Medica
2025;50(3):583-589
- CountryChina
- Language:Chinese
-
Abstract:
Osteoporosis(OP) is a senile bone disease characterized by an imbalance between bone remodeling and bone formation. Targeting pathogenesis of kidney deficiency, spleen deficiency, and blood stasis, Bushen Jianpi Huoxue Decoction has a significant effect on the treatment of OP by tonifying kidney, invigorating spleen, and activating blood circulation. MicroRNA(miRNA) and the anti-apoptotic protein B-cell lymphoma-2-like protein 1(BCL2L1) are closely related to bone cell metabolism. Therefore, in this study, the binding of miR-140-5p to BCL2L1 was detected by dual luciferase assay and polymerase chain reaction(PCR). After silencing or overexpressing miR-140-5p, the apoptosis, autophagy, and osteogenic function of human fetal osteoblast cell line 1.19(HFOB1.19) were observed by flow cytometry and Western blot. Bushen Jianpi Huoxue Decoction-containing serum was prepared by intragastric administration of Bushen Jianpi Huoxue Decoction in rats. Different concentrations of Bushen Jianpi Huoxue Decoction-containing serum were used to treat HFOB1.19 with or without miR-140-5p mimic. The expression of osteogenic proteins in each group was observed, and the role of miR-140-5p/BCL2L1 in apoptosis and autophagy of HFOB1.19 was studied, along with the effect of Bushen Jianpi Huoxue Decoction on these processes. As indicated by the dual luciferase assay, miR-140-5p bound to BCL2L1. Flow cytometry and Western blot showed that miR-140-5p promoted apoptosis and inhibited autophagy in HFOB1.19. After intervention with high, medium, and low doses of Bushen Jianpi Huoxue Decoction-medicated serum, compared with the miR-140-5p NC group, the expression of osteocalcin(OCN), osteopontin(OPN), Runt-related transcription factor 2(RUNX2), and transforming growth factor beta 1(TGF-β1) decreased in the miR-140-5p mimic group, while the expression of bone morphogenetic protein 2(BMP2) showed no significant difference under high-dose intervention. Therefore, miR-140-5p/BCL2L1 can promote apoptosis and inhibit autophagy in HFOB1.19. Bushen Jianpi Huoxue Decoction can affect the osteogenic effect of miR-140-5p through BMP2.
