One-year seedling cultivation technology and seed germination-promoting mechanism by warm water soaking of Polygonatum kingianum var. grandifolium.
10.19540/j.cnki.cjcmm.20241115.101
- Author:
Ke FU
1
;
Jian-Qing ZHOU
2
;
Zhi-Wei FAN
3
;
Mei-Sen YANG
4
;
Ya-Qun CHENG
5
;
Yan ZHU
1
;
Yan SHI
1
;
Jin-Ping SI
1
;
Dong-Hong CHEN
1
Author Information
1. National Innovation Alliance of Huangjing Industry, State Key Laboratory of Subtropical Silviculture, Zhejiang A&F University Hangzhou 311300, China.
2. Ecological Forestry Development Center of Jingning She Autonomous County Lishui 323500, China.
3. National Innovation Alliance of Huangjing Industry, State Key Laboratory of Subtropical Silviculture, Zhejiang A&F University Hangzhou 311300, China Guizhou Botanical Garden Guiyang 550004, China.
4. Xiushan Traditional Chinese Medicine Industry Center Chongqing 409000, China.
5. Chongqing Hexin Agricultural Technology Co., Ltd. Chongqing 409000, China.
- Publication Type:Journal Article
- Keywords:
Polygonati Rhizoma;
cultivation temperature;
one-year seedling cultivation;
sand storage at low temperatures;
seed germination;
warm water soaking
- MeSH:
Germination;
Seedlings/genetics*;
Water/metabolism*;
Seeds/metabolism*;
Polygonatum/genetics*;
Temperature;
Plant Proteins/genetics*;
Plant Dormancy
- From:
China Journal of Chinese Materia Medica
2025;50(4):1022-1030
- CountryChina
- Language:Chinese
-
Abstract:
Polygonati Rhizoma demonstrates significant potential for addressing both chronic and hidden hunger. The supply of high-quality seedlings is a primary factor influencing the development of the Polygonati Rhizoma industry. Warm water soaking is often used in agriculture to promote the rapid germination of seeds, while its application and molecular mechanism in Polygonati Rhizoma have not been reported. To rapidly obtain high-quality seedlings, this study treated Polygonatum kingianum var. grandifolium seeds with sand storage at low temperatures, warm water soaking, and cultivation temperature gradients. The results showed that the culture at 25 ℃ or sand storage at 4 ℃ for 2 months rapidly broke the seed dormancy of P. kingianum var. grandifolium, while the culture at 20 ℃ or sand storage at 4 ℃ for 1 month failed to break the seed dormancy. Soaking seeds in 60 ℃ warm water further increased the germination rate, germination potential, and germination index. Specifically, the seeds soaked at 60 ℃ and cultured at 25 ℃ without sand storage treatment(Aa25) achieved a germination rate of 78. 67%±1. 53% on day 42 and 83. 40%±4. 63% on day 77. The seeds pretreated with sand storage at 4 ℃ for 2 months, soaked in 60 ℃ water, and then cultured at 25 ℃ achieved a germination rate comparable to that of Aa25 on day 77. Transcriptomic analysis indicated that warm water soaking might promote germination by triggering reactive oxygen species( ROS), inducing the expression of heat shock factors( HSFs) and heat shock proteins( HSPs), which accelerated DNA replication, transcript maturation, translation, and processing, thereby facilitating the accumulation and turnover of genetic materials. According to the results of indoor controlled experiments and field practices, maintaining a germination and seedling cultivation environment at approximately 25 ℃ was crucial for the one-year seedling cultivation of P. kingianum var. grandifolium.
