Prediction of quality markers for cough-relieving and phlegm-expelling effects of Kening Granules based on plasma pharmacology combined with network pharmacology and pharmacokinetics.
10.19540/j.cnki.cjcmm.20241218.201
- Author:
Qing-Qing CHEN
1
;
Yuan-Xian ZHANG
1
;
Qian WANG
1
;
Jin-Ling ZHANG
1
;
Lin ZHENG
2
;
Yong HUANG
3
;
Yang JIN
3
;
Zi-Peng GONG
2
;
Yue-Ting LI
3
Author Information
1. State Key Laboratory of Functions and Applications of Medicinal Plants, Engineering Research Center for the Development and Application of Ethnic Medicine and TCM (Ministry of Education), National Engineering Research Center of Miao's Medicines,Guizhou Medical University Guiyang 550004, China School of Pharmaceutical Sciences, Guizhou Medical University Guiyang 550004, China.
2. Guizhou Provincial Key Laboratory of Pharmaceutics, Guizhou Medical University Guiyang 550004, China.
3. State Key Laboratory of Functions and Applications of Medicinal Plants, Engineering Research Center for the Development and Application of Ethnic Medicine and TCM (Ministry of Education), National Engineering Research Center of Miao's Medicines,Guizhou Medical University Guiyang 550004, China.
- Publication Type:Journal Article
- Keywords:
Kening Granules;
cough-relieving and phlegm-expelling effects;
network pharmacology;
quality marker
- MeSH:
Drugs, Chinese Herbal/administration & dosage*;
Network Pharmacology;
Cough/blood*;
Male;
Humans;
Animals;
Rats;
Rats, Sprague-Dawley;
Biomarkers/blood*;
Quality Control;
Chromatography, High Pressure Liquid;
Antitussive Agents/chemistry*
- From:
China Journal of Chinese Materia Medica
2025;50(4):959-973
- CountryChina
- Language:Chinese
-
Abstract:
This study predicts the quality markers(Q-markers) for the cough-relieving and phlegm-expelling effects of Kening Granules based on pharmacodynamics, plasma drug chemistry, network pharmacology, and pharmacokinetics. Strong ammonia solution spray and phenol red secretion assays were employed to evaluate the cough-relieving and phlegm-expelling effects of Kening Granules. Twentysix absorbed prototype components of Kening Granules were identified by ultra high performance liquid chromatography coupled with QExactive Plus quadrupole/Orbitrap high resolution mass spectrometry(UHPLC-Q-Exactive Plus Orbitrap HRMS). Through network pharmacology, 11 potential active components were screened out for the cough-relieving and phlegm-expelling effects of Kening Granules. The 11 components acted on 40 common targets such as IL6, TLR4, and STAT3, which mainly participated in PI3K/Akt, HIF-1, and EGFR signaling pathways. Pharmacokinetic quantitative analysis was performed for 7 prototype components. Three compounds including azelaic acid, caffeic acid, and vanillin were identified as Q-markers for the cough-relieving and phlegm-expelling effects of Kening Granules based on their effectiveness, transmissibility, and measurability. The results of this study are of great significance for clarifying the pharmacological substance basis, optimizing the quality standards, and promoting the clinical application of Kening Granules.
