Angelicae Dahuricae Radix polysaccharides treat ulcerative colitis in mice by regulating gut microbiota and metabolism.
10.19540/j.cnki.cjcmm.20241014.703
- Author:
Feng XU
1
;
Lei ZHU
2
;
Ya-Nan LI
2
;
Cheng CHENG
2
;
Yuan CUI
2
;
Yi-Heng TONG
2
;
Jing-Yi HU
2
;
Hong SHEN
2
Author Information
1. Institute of Digestive Diseases, Affiliated Hospital of Nanjing University of Chinese Medicine Nanjing 210029, China Department of Gastroenterology, the Third Affiliated Hospital of Zhejiang Chinese Medical University Hangzhou 310005, China.
2. Institute of Digestive Diseases, Affiliated Hospital of Nanjing University of Chinese Medicine Nanjing 210029, China Department of Gastroenterology, Jiangsu Province Hospital of Chinese Medicine Nanjing 210029, China.
- Publication Type:Journal Article
- Keywords:
Angelicae Dahuricae Radix polysaccharides;
gut microbiota;
metabolomics;
ulcerative colitis
- MeSH:
Animals;
Gastrointestinal Microbiome/drug effects*;
Colitis, Ulcerative/genetics*;
Mice;
Male;
Drugs, Chinese Herbal/administration & dosage*;
Polysaccharides/administration & dosage*;
Angelica/chemistry*;
Humans;
Colon/metabolism*;
Disease Models, Animal;
Mucin-2/metabolism*;
Tumor Necrosis Factor-alpha/metabolism*
- From:
China Journal of Chinese Materia Medica
2025;50(4):896-907
- CountryChina
- Language:Chinese
-
Abstract:
This study employed 16S r RNA gene high-throughput sequencing and metabolomics to explore the mechanism of Angelicae Dahuricae Radix polysaccharides(RP) in the treatment of ulcerative colitis(UC). A mouse model of UC was induced with 2. 5% dextran sulfate sodium. The therapeutic effects of RP on UC in mice were evaluated based on changes in body weight, disease activity index( DAI), and colon length, as well as pathological changes. RT-qPCR was performed to assess the m RNA levels of interleukin(IL)-6, IL-1β, tumor necrosis factor(TNF)-α, myeloperoxidase(MPO), mucin 2(Muc2), Occludin, Claudin2, and ZO-1 in the mouse colon tissue. ELISA was employed to measure the expression of IL-1β and TNF-α in the colon tissue. The intestinal permeability of mice was evaluated by the fluorescent dye permeability assay. Immunohistochemistry was employed to detect the expression of Muc2 and occludin in the colon tissue. Changes in gut microbiota and metabolites were analyzed by 16S r RNA sequencing and ultra-high-performance liquid chromatography coupled with quadrupole-orbitrap mass spectrometry( UPLC-Q-Exactive Plus Orbitrap MS), respectively. The results indicated that low-dose RP alleviated general symptoms, reduced colonic inflammation and intestinal permeability, and promoted Muc2 secretion and tight junction protein expression in UC mice. In addition, low-dose RP increased gut microbiota diversity in UC mice and decreased the relative abundance of harmful bacteria such as Ochrobactrum and Streptococcus. Twenty-seven differential metabolites were identified in feces, and low-dose RP restored the levels of disturbed metabolites. Notably, arginine and proline metabolism were the most significantly altered amino acid metabolic pathways following lowdose RP intervention. In conclusion, RP can ameliorate general symptoms, inhibit colonic inflammation, and maintain intestinal mucosal barrier integrity in UC mice by modulating gut microbiota composition and arginine and proline metabolism.
