Chemical constituents from Alternaria alternata and their activity of down-regulating TMSB10 expression.
10.19540/j.cnki.cjcmm.20241008.201
- Author:
Lan WANG
1
;
Nuan ZHANG
2
;
Tao YUAN
1
Author Information
1. School of Health, Jiangxi Normal University Nanchang 330022, China College of Life Sciences, Jiangxi Normal University Nanchang 330022, China.
2. School of Health, Jiangxi Normal University Nanchang 330022, China.
- Publication Type:Journal Article
- Keywords:
Alternaria alternata;
TMSB10;
benzopyrone;
non-small cell lung cancer
- MeSH:
Alternaria/metabolism*;
Down-Regulation/drug effects*;
Humans;
Molecular Structure
- From:
China Journal of Chinese Materia Medica
2025;50(1):139-156
- CountryChina
- Language:Chinese
-
Abstract:
This study investigated the secondary metabolites in the product of rice fermentation with Alternaria alternata and their activity of down-regulating thymosin beta 10(TMSB10) protein expression. The secondary metabolites of A. alternata were separated and purified by various chromatographic separation techniques, including silica gel column chromatography, Sephadex LH-20 column chromatography, and semi-preparative high-performance liquid chromatography. Their structures were identified by spectral techniques such as nuclear magnetic resonance spectroscopy(NMR), infrared spectroscopy(IR), and high-resolution electrospray ionization mass spectrometry(HR-ESI-MS) and comparison with literature data. A total of 10 compounds were isolated and identified, including two new compounds(1S,3S)-2,3-dihydro-3,6-dihydroxy-8-methoxy-1-methylcyclopenta[c][2]benzopyran-5(1H)-one(1), 3,3a, 6-trihydroxy-8-methoxy-1-methyl-2,3,3a, 9b-tetrahydrocyclopenta[c]isochromen-5(1H)-one(2), and the eight known compounds are alternariol 9-methyl ether(3), 1-deoxyrubralactone(4),(3aR)-3,3a-dihydro-1,6-dihydroxy-8-methoxy-3a-methylcyclopenta[c][2]benzopyran-2,5-dione(5), altechromone A(6), alternariol(7), altenuene(8), altenusin(9), and 3'-hydroxyalternariol 5-O-methyl ether(10). The effects of these compounds on TMSB10 expression were tested. Compound 7 showed a significant down-regulation effect on TMSB10 expression with an inhibition rate of 40.5%. The results showed that benzopyrone compounds of A. alternata have the activity of down-regulating the expression of TMSB10 protein, providing theoretical basis and research value for the study of non-small cell lung cancer.
