Pharmacokinetics study of Dayuanyin in normal and febrile rats.
10.19540/j.cnki.cjcmm.20241010.201
- Author:
Yu-Jie HOU
1
;
Kang-Ning XIAO
2
;
Jian-Yun BI
3
;
Xin-Jun ZHANG
2
;
Xin-Rui LI
2
;
Yu-Qing WANG
2
;
Ming SU
2
;
Xin-Ru SUN
2
;
Hui ZHANG
2
;
Bo-Yang WANG
2
;
Li-Jie WANG
3
;
Shan-Xin LIU
4
Author Information
1. Shangdong Academy of Chinese Medicine Ji'nan 250014, China Shandong University of Traditional Chinese Medicine Ji'nan 250355, China.
2. Shangdong Academy of Chinese Medicine Ji'nan 250014, China.
3. Shandong University of Traditional Chinese Medicine Ji'nan 250355, China.
4. Shangdong Academy of Chinese Medicine Ji'nan 250014, China Shandong Key Laboratory of Basic Theoretical Innovation and Applied Research of Traditional Chinese Medicine Ji'nan 250355, China.
- Publication Type:Journal Article
- Keywords:
Dayuanyin;
albiflorin;
paeoniflorin;
pharmacokinetics;
wogonin;
wogonoside
- MeSH:
Animals;
Rats;
Drugs, Chinese Herbal/administration & dosage*;
Male;
Rats, Sprague-Dawley;
Fever/metabolism*;
Tandem Mass Spectrometry;
Chromatography, High Pressure Liquid;
Glucosides/pharmacokinetics*;
Monoterpenes
- From:
China Journal of Chinese Materia Medica
2025;50(2):527-533
- CountryChina
- Language:Chinese
-
Abstract:
Based on the pharmacokinetics theory, this study investigated the pharmacokinetic characteristics of albiflorin, paeoniflorin, wogonoside, and wogonin in normal and febrile rats and summarized absorption and elimination rules of Dayuanyin in them to provide reference for further development and clinical application of Dayuanyin. Blood samples were taken from the fundus venous plexus of normal and model rats after intragastric administration of Dayuanyin at different time points. The concentration of each substance in blood was determined by ultra performance liquid chromatography-triple quadrupole mass spectrometry(UPLC-MS/MS) technique at different time points. DAS 2.0, a piece of pharmacokinetics software, was used to calculate the pharmacokinetic parameters of each component. The results show that the 4 components had good linear relationship in their respective ranges, and the results of methodological investigation met the requirements. The pharmacokinetic parameters of C_(max), T_(max), t_(1/2), AUC_(0-t), AUC_(0-∞), and MRT_(0-t) were calculated by the DAS 2.0 non-compartmental model. Compared with those in the normal group, C_(max) and AUC_(0-t) of the 4 components in the model group were significantly increased. There were significant differences in the pharmacokinetic characteristics between the normal and model groups, suggesting that the absorption and elimination of Dayuanyin may be affected by the changes of internal environment of the body in different physiological states.
