Chemical constituents of butyl-phthalides from Ligusticum sinense.
10.19540/j.cnki.cjcmm.20241101.202
- Author:
Hang LIU
1
;
Xue-Ming ZHOU
1
;
Ting ZHENG
1
;
Mei-Zhu WU
1
;
Shuo FENG
1
;
Ye LIN
1
;
Xin-Ming SONG
1
;
Ji-Ling YI
1
Author Information
1. Key Laboratory of Tropical Medicinal Resources Chemistry of Ministry of Education, Key Laboratory of Tropical Medicinal Plant Chemistry of Hainan Province, Hainan Normal University Haikou 571158, China.
- Publication Type:Journal Article
- Keywords:
Ligusticum sinense;
anti-inflammatory activity;
butyl-phthalides;
chemical constituents
- MeSH:
Animals;
Mice;
Nitric Oxide/biosynthesis*;
Ligusticum/chemistry*;
Benzofurans/isolation & purification*;
Drugs, Chinese Herbal/isolation & purification*;
Macrophages/immunology*;
RAW 264.7 Cells;
Molecular Structure
- From:
China Journal of Chinese Materia Medica
2025;50(2):439-443
- CountryChina
- Language:Chinese
-
Abstract:
Eight butyl-phthalides, senkyunolide K(1), senkyunolide N(2), butylphthalide(3), senkyunolide I(4), senkyunolide H(5),(Z)-butylidenephthalide(6),(Z)-ligustilide(7), and 3-butylidene-7-hydroxyphthalide(8) were isolated from the aerial part of Ligusticum sinense by column chromatography on silica gel column, ODS, Sephadex LH-20 and semi-preparative HPLC. Their structures were elucidated on the basis of spectroscopic and chemical data, especially NMR and MS. Compound 1 was a new butyl-phthalide and compounds 2-8 were isolated from the aerial part of L. sinense for the first time. Furthermore, the inhibitory activities of compounds 1-8 against the nitric oxide(NO) production induced by lipopolysaccharide(LPS) in mouse RAW264.7 macrophages in vitro were evaluated. The results showed that compounds 1-8 exerted inhibitory activities on NO production with IC_(50) of 19.34-42.16 μmol·L~(-1).
