Identification of GSK3 family and regulatory effects of brassinolide on growth and development of Nardostachys jatamansi.
10.19540/j.cnki.cjcmm.20241011.102
- Author:
Yu-Yan LEI
1
;
Zheng MA
2
;
Jing WEI
1
;
Wen-Bing LI
3
;
Ying LI
3
;
Zheng-Ming YANG
3
;
Shao-Shan ZHANG
3
;
Jing-Qiu FENG
3
;
Hua-Chun SHENG
3
;
Yuan LIU
3
Author Information
1. College of Pharmacy, Southwest Minzu University Chengdu 610041, China Sichuan Technology and Engineering Laboratory of Qiang-Yi Medicinal Resources Protection and Utilization Chengdu 610225, China Key Laboratory of Tibetan Plateau Ethnic Medicinal Resources Protection and Utilization of National Ethnic Affairs Commission Chengdu 610225, China.
2. College of Pharmacy, Southwest Minzu University Chengdu 610041, China.
3. Sichuan Technology and Engineering Laboratory of Qiang-Yi Medicinal Resources Protection and Utilization Chengdu 610225, China Key Laboratory of Tibetan Plateau Ethnic Medicinal Resources Protection and Utilization of National Ethnic Affairs Commission Chengdu 610225, China.
- Publication Type:Journal Article
- Keywords:
BIN2;
GSK3;
Nardostachys jatamansi;
RT-qPCR
- MeSH:
Brassinosteroids/metabolism*;
Steroids, Heterocyclic/metabolism*;
Gene Expression Regulation, Plant/drug effects*;
Plant Proteins/metabolism*;
Phylogeny;
Nardostachys/metabolism*;
Plant Growth Regulators/pharmacology*;
Plant Roots/drug effects*
- From:
China Journal of Chinese Materia Medica
2025;50(2):395-403
- CountryChina
- Language:Chinese
-
Abstract:
This study identified 8 members including NjBIN2 of the GSK3 family in Nardostachys jatamansi by bioinformatics analysis. Moreover, the phylogenetic tree revealed that the GKS3 family members of N. jatamansi had a close relationship with those of Arabidopsis. RT-qPCR results showed that NjBIN2 presented a tissue-specific expression pattern with the highest expression in roots, suggesting that NjBIN2 played a role in root growth and development. In addition, the application of epibrassinolide or the brassinosteroid(BR) synthesis inhibitor(brassinazole) altered the expression pattern of NjBIN2 and influenced the photomorphogenesis(cotyledon opening) and root development of N. jatamansi, which provided direct evidence about the functions of NjBIN2. In conclusion, this study highlights the roles of BIN2 in regulating the growth and development of N. jatamansi by analyzing the expression pattern and biological function of NjBIN2. It not only enriches the understanding about the regulatory mechanism of the growth and development of N. jatamansi but also provides a theoretical basis and potential gene targets for molecular breeding of N. jatamansi with improved quality in the future.
